Modulatory effect of Urera aurantiaca extract on immune and tumoral cells during inflammation

ANESINI CLAUDIA; MARRASSINI CARLA

Mar del Plata

Congreso; XLVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (SAFE); 2016

SAFE

There is a known link between inflammation and cancer. Urera aurantiaca (Urticaceae) is a medicinal plant used in inflammation. The effect of a methanol extract (UA) upon the viability (MTT method) and proliferation (thymidine tritiated uptake) of normal and tumoral lymphocytes under the effect of prostaglandin E2 (PGE2), and on nitric oxide (NO) production by LPS-stimulated macrophages (Griess method) was analyzed. PGE2 mechanism of action was studied in presence of inhibitors: staurosporine (ST) 10-9M (PKC inhibitor), SB 203580 10mM (MAPK P38 inhibitor), PD 98059 5µM (ERK1/2 inhibitor), sodium azide (SA) 2mM (peroxidase inhibitor). Results are expressed as fold of stimulation respect to basal (Mean±SEM) of 3 experiments made by triplicate (*p