Antioxidant activity of Larrea divaricata loaded in mucoadhesive polymers and silica composites

C.E. OLIVETTI; R. ALONSO; M.F. DESIMONE; M.I. ALVAREZ ECHAZÚ; O. PERNA; G.S. ALVAREZ; I. PERALTA; C. ANESINI

Córdoba

Congreso; Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2016

TÍTULOAntioxidant activity of Larrea divaricata loaded in mucoadhesive polymers and silica compositesAUTORESM.I. Alvarez Echazú, C.E. Olivetti, I. Peralta, O. Perna, R. Alonso, C. Anesini, G.S. Alvarez, M.F. DesimoneLUGAR DE TRABAJOUniversidad de Buenos Aires. CONICET. IQUIMEFA. Facultad de Farmacia y Bioquímica, ArgentinaRESUMENOral diseases therapy has been monopolized for years with clorhexidine, triclosan and essential oils because of their good clinical results. Nevertheless, new alternatives need to be explored to prevent and treat gum disease. Besides, the ongoing knowledge in biomaterials has allowed to a high versatility in clinical approaches in dental field. Biodegradable scaffolds, such as chitosan, have an important role in oral tissue regeneration. Additionally, they are employed as drug delivery systems.Chitosan-silica composites and chitosan-CMC-silica composites were plasticized with glycerol. They were characterized by SEM and FTIR. Both techniques revealed the presence of silica in chitosan polymer matrix. These composites loaded with L. divaricata extract, present antioxidant properties. Indeed, DPPH scavenger activity (%) was CMC-SiO2: 30.8 ± 1.2 and SiO2: 19.5 ± 2.2 and SOD (adrenaline oxidation % of inhibition) was CMC-SiO2: 17.1 ± 0.120 and SiO2: 5.3 ± 2.1. HPLC analysis demonstrated that polar compounds were incorporated to the composites but NDGA, a majority low polar compound found in the extract, was not incorporated in both composites. CMC-SiO2 composites exerted a higher antioxidant activity than SiO2 in agreement with higher extract incorporation. Scaffolds loaded with L. divaricata have promising antioxidant and anti-inflammatory characteristics suitable for dental care.