IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Arterial blood pressure increase and renal alterations induced by moderate zinc deficiency during fetal and postnatal
Autor/es:
ARRANZ CRISTINA; TOMAT ANALIA; VEIRAS LUCIANA; FINELLA SEBASTIÁN; LOPEZ FERRUCCI DÉBORA; INSERRA FELIPE; BALASZCZUK ANA MARÍA; COSTA MARIA DE LOS ANGELES
Lugar:
Berlín. Alemania.
Reunión:
Congreso; Eighteenth European Meeting on Hypertension.; 2008
Institución organizadora:
European Society of Hypertension. International Society of Hypertension.
Resumen:
Objectives: We have showed that moderate zinc deficiency during fetal life, lactation and/or post-weaning growth induces an increase in systolic blood pressure (SBP)and an impaired renal function in the adult life, associated with a reduction in the number and size of glomeruli. Our objective was to study the possible mechanisms involved in these renal and cardiovascular effects induced by moderate zinc deficiency during fetal, early postnatal life and/or post-weaning growth. Methods: Wistar rats received from the beginning of pregnancy up to weaning low (8 ppm) or control (30 ppm) zinc diet. After weaning, male offspring of each group of mothers continued with low (Cl and Ll) or control (Cc and Lc) zinc diet during 60 days. At the end of the experimental period, we determined: 24 hs-urinary proteins, proapoptotic protein (Bax) and antiapoptotic protein (Bcl-2), signs of fibrosis; thiobarbituric acid-reactive substances (TBARS) and glutathione concentration, glutathione reductase activity (GPx) in renal tissue. Results: Sirius Red and a-SMA staining showed signs of fibrotic process in glomeruli, tubules and peritubular interstitium of Ll and Lc kidneys.*p<0.01 vs Cc; +p<0.05 vs Cl; #p<0.05 vs Lc. Cc Cl Lc Ll Urinary proteins (mg/24hs.100g) 3.09±0.86 2.73±0.54 6.26±0.74*+ 14.5±2.09*#+ TBARS (nmol/mg.protein) 0.16±0.02 0.46±0.02* 0.38±0.05* 0.66±0.08*#+ Glutathione (mg/mg protein) 2.10±0.2 1.40±0.02*# 1.9±0.05 1.30±0.05*# GPx activity (pmol/min.mg.protein) 8.6±1.8 3.5±0.9*# 6.4±0.05* 3.1±0.03*# Ratio Bax/Bcl-2 1.3±0.1 3.9±0.4* 3.5±0.3* 2.9±0.04* SBP (mmHg) 120±3 147±4* 145±4* 146±5* Conclusion: The higher oxidative stress levels and the activation of apoptotic and fibrotic processes in the kidney, could explain, at least in part, the increase of arterial blood pressure and the reduction of renal function and filtration surface area, observed in this model of moderate zinc deficiency.