IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Stability of an aqueous extract Larrea divaricata cav during a simulated digestion process
Autor/es:
RENZO MARTINO; IGNACIO PERALTA; MARÍA ROSARIO ALONSO; DEMIAN MONTI; CLAUDIA ANESINI
Lugar:
Mar del Plata
Reunión:
Congreso; XLVIII REUNION ANUAL DE LA SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (SAFE); 2016
Institución organizadora:
Sociedad Argentina de Farmacología Experimental
Resumen:
Medicinal plants are used as therapeutic agents but little is known about their pharmacokinetic properties especially the stability in gastric and intestinal fluids, phenomenon that affects their bioavailability. Solubility and stability in gastrointestinal fluids is normally a prerequisite for the potential in vivo beneficial role of an extract given by an oral route. Larrea divaricata Cav. is a South American plant widely distributed in Argentina, which aqueous extract exerts antioxidant, antitumoral and antimicrobial activities. Nevertheless, nothing is known about its stability in simulated digestion fluids. So the aim of this work was to study the stability but also the solubility of a lyophilized aqueous extract of this plant compressed as a pill. In order to do this, quantification of its majority polyphenol compound nor-dihydroguaiaretic acid (NDGA), of the total polyphenols and flavonoids as well as the antioxidant activity parameters such as DPPH scavenger activity and reducing power were assayed after submitting the extract to different incubations time in simulated digestive fluids.The percentage of recovery of NDGA and total polyphenols was high, in the order of 90% in all fluids. Total flavonoids slightly decreased after incubation in gastric fluid (60 min and 120 min) from 12 to 18 % respectively. On the contrary incubations in intestinal fluid during 120 and 240 min provoked a higher decrease than in gastric fluids reaching a value of 45 % and 40 % respectively. The DPPH scavenger activity as well as the reducing power decreased in intestinal fluids 12% or 14 % respectively.These results suggested that NDGA did not change its solubility depending on pH but also it didn?t suffer any metabolism in presence of enzymes or pH variance. Polyphenols, such as NDGA, could balance out the activity in view of flavonoids drop. We concluded that the extract was stable in gastrointestinal simulated fluids maintaining its antioxidant activities which could support its use by an oral route.