IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of silica nanoparticles (Si02NPs) on Monocytes/Macrophages cells
Autor/es:
SARACENO M; MITAROTONDA R; TODONE M; FERNANDEZ M; MALCHIODI EL; DESIMONE MF; DE MARZI MC
Lugar:
Buenos Aires
Reunión:
Congreso; SETAC Latin America 11th Biennial Meeting; 2015
Institución organizadora:
Society of Environmental Toxicology and Chemistry
Resumen:
The employment of nanoparticles (NPs) as drug delivery has gained attraction in recent years for their use with therapeutic purposes. NPs can also be used as a carrier for immunostimulatory or immunosuppressive molecules. In addition, NPs can transport antigens to generate a specific immune response. Recently, silica NPs (SiO2NPs) has proved to be a suitable and biocompatible tool for the immobilization of biomolecules, microorganisms, and for the entrapment of mammals? cells. In this work we obtained SiO2NPs of different size (10-500 nm, as determined by Dynamic Light Scattering) and charge (positives and negatives, as zeta potential characterization) by the Stöber method or using inverse microemulsions and surface modification. We analyzed the effect of the different NPs on monocytes/macrophages cultures (THP-1) at different times (24-168 h), cells densities (0.1-1 x 106 cells/ml) and NPs concentrations (10-100 nM). Proliferation of cells were determined by MTT assay observing that different positive NPs did not affected cell proliferation while negative NPs ≥ 380 nm decreased between 50-70% THP-1 cells proliferation at 24 h of culture, and negative NPs between 100-380 nm need at least 48 h of culture to reduce cell proliferation 32-83 % respect to controls. At 168 h of culture all negative NPs reduce proliferation ~30-40%. On the other way, cell activation was evaluated. Negative NPs ≥100 nm increased nitrite secretion by 24 h of culture (8-16 times). In the same way negative NPs ≥ 100 nm increase IL8 and IL12 secretion by 48 h (2-10 times respect controls). Positive NPs and 10 nm negative NPs don?t induce ILs or nitrite secretion. All negative NPs increased membrane expression of CD86, CD80 and CD14 (~2-8 times) while positive ones increased CD86 and CD14 expression (2-10 times). Negative NPs decreased CD11 expression (~50%). Finally, negative NPs increase monocyte/macrophage membrane damage after 48 h of culture as determined by 7AAD cell incorporation (1.5-4 times). We detect a low percentage of cells in apoptosis process. In conclusion, negative silica NPs ≥ 100 nm have cell activation properties, and could be useful for immune stimulation, while negative NPs of 10 nm and positive NPs have low impact on immune system cells and would be useful for molecule transportation since they are unable to stimulate antigen presenting cells.