IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of SiO2NPs on blood components
Autor/es:
MITAROTONDA R; DE MARZI MC; DESIMONE MF
Lugar:
Buenos Aires
Reunión:
Congreso; SETAC Latin America 11th Biennial Meeting; 2015
Institución organizadora:
Society of Environmental Toxicology and Chemistry
Resumen:
In recent years, silica nanoparticles (SiO2NPs) have gained attention because of its potential use for diagnosis or drug delivery. However, if nanoparticles (NPs) get in contact with blood components, different molecules (protein, lipids, carbohydrates, etc.) could be adsorbed on its surface modifying its behavior. Based on previously described, the aim of this work is to analyze the interaction between SiO2NPs and blood components. Negative SiO2NPs were synthesized using the Stöber method. Positive NPs were obtained after APTES treatment. Size and morphology were assessed using a transmission electron microscope. The hydrodynamic diameter and zeta potential of NPs were characterized by dynamic light scattering. After NPs-serum incubation, we observed, by SDS-PAGE and Bradford method, the adsorption capacity of several human serum proteins (~6mg/ml) by all SiO2NPs (100 mM). The adsorption remained stable during at least 9 days. In order to known the hemolytic behaviors of NP, human red blood cells (RBC) were incubated with NPs (10 mM) and hemoglobin released (DO 540 nm) were determinate as indicator of RBC hemolysis. We determinate that negative SiO2NPs previously treated with human serum decrease the RBC hemolysis (2.6-4.1 % hemolysis) respect negative SiO2NPs only (81.8-86.4 % hemolysis). On the other hand, positive NPs do not affect RBC (0-8 % hemolysis). For study the effect of NP on coagulation the partial thromboplastin time (aPPT) was determined using a semi-automatic blood coagulation analyzer. We observed that all SiO2NPs act as anticoagulants (12-53s) while this effect decreases with SiO2NPs previously treated with human serum (0.1-26 s). Because the importance of calcium in coagulation, we determine its concentration adsorbed on the NPs. The concentration was higher in the negative NPs treated or not with human serum respects positive NPs. In this work, we characterized and evaluated interaction of silica nanoparticles with blood components. These preliminary results allow us to continue with the studies with other cellular component in circulation to check the potential usefulness of these nanoparticles for drug delivery.