Effect of mesoporous silica nanoparticles on SAOS viability and mesenchymal cells differentiation

ALVAREZ GISELA; ALVAREZ ECHAZU, MI; OLIVETTI CHRISTIAN; DESIMONE MARTIN

Encuentro; SETAC Latinoamerican 11th biennial meeting; 2015

The effect of various mesoporous silica nanoparticle concentrations on SAOS viability was determined in vitro. These nanoparticles significantly increased cell proliferation after 48 h compared to a control in concentrations between 0.06 and 0.6 mg ml-1. However, nanoparticles in concentrations above 1.2 mg ml-1 appeared to be toxic for cell with a 40 % decrease in their viability for 2.4 mg ml-1. These results are in agreement with previous works where rat primary culture osteoblasts in contact with the ionic products from the dissolution of a bioactive glass with 60% of silica, increased their viability when compared to control cultures. Alkaline phosphatase activity was measured after incubating mesenchymal cells with MNP. Interestingly, MNP also had a differentiation effect over cells as ALP activity significantly increased.