IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
In vivo biocompatibility evaluation of gentamicin silica-collagen nanocomposites
Autor/es:
A. M. MEBERT; G. S. ALVAREZ; C. HÉLARY; T. CORADIN; M. F. DESIMONE
Reunión:
Congreso; LI Reunión SAIB 2015; 2015
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Resumen:
Abstract: Nanocomposites are composites in which at least one of the phases shows nanometric dimensions. In this work we evaluated in vivo biocompatibility of Silica nanoparticle-collagen I hydrogels composites as gentamicin releasing systems for medical applications, that showed prolonged antibacterial activity in a previous work. One step loaded 500nm silica nanoparticles (SiNPs) were synthesized according to the Stöber method. For this purpose Tetraethyl orthosilicate (TEOS) and ammonium hydroxide were added to a water-ethanol mixture with or without (blank) gentamicin sulphate. Monodisperse drug free and gentamicin loaded SiNPs were observed by scanning electron microscopy (SEM). Collagen type I was purified from rat tails and nanocomposites were prepared by mixing 30mg SiNPs with 5mg/mL collagen suspension (0,5M Si). Antibacterial activity of gentamicin SiNPs (338 IU per g NPs) were evaluated by the disk diffusion method as described in the US Pharmacopeia. Pseudomonas aeruginosa was used as sensitive microorganism and LB agar as growth medium. Collagen hydrogel and nanocomposites morphology were also observed by SEM. Biocompatibility was evaluated in vivo by implanting subcutaneously 300uL hydrogel or composite on Wistar rats (250g). No clear toxicity was observed by histology and immunohistological studies after 15 days.