Erythropoietin therapy modifies cardiovascular adaptative response in haemorrhaged rats.

PUCHULU MB; ARRECHE ND; ALTALEF R; OGONOWSKI N; FELLET A; BALASZCZUK AM

Simposio; Network for the Advacement of Patient Blood Managment Haemostasis and Thrombosis. 16th Annual Symposium; 2015

Introduction: Acute haemorrhage can lead haemodynamic instability andtissue perfusion resulting in multiple organ failure. Erythropoietin (EPO) is aglycoprotein hormone produced by the kidney and plays a key role inhematopoiesis.  In addition to thesehematopoietic effects, EPO exerts non-hematopoietic effects on cardiovascularsystem. The aim of the present study was to investigate the pre-treatment withEPO modifies the cardiovascular pareameters in acute haemorrhage. Methods: Male Sprague- Dawley rats, 2months old were divided into: control + PBS 3-day pre-treatment (C);haemorrhage animals (H) + PBS 3 day pre-treatment; EPO 3 day pre-treatment  and H + EPO 3 day pre-treatment. Animals weretreated with either 1 ml/kg PBS or 1000 UI/kg recombinant human EPO i.p dailyfor 3 days. On day 4, rats were subjected to surgical procedure only orhaemorrhage (20 % blood loss).  Meanarterial pressure (MAP) and heart rate (HR) were evaluated during 120 min. Results: EPO pre-treatment did notaffect MAP and HR basal values. Hemorrhage induced a marked decrease in MAP inH group which reach a value of  22 ± 4mmHgat 5 min following the bleeding period (*p<0.01vs basal values) withsubsequent stabilization at about 54 ± 5 mmHg at 15 min (*p<0.01vs basal values).In H + EPO group, the haemorrhage induced a decrease in MAP at 5 min (43 ± 5mmHg, *p<0.01 vs basal values) but MAP values increased reaching at 60 min baselinevalues (75 ± 3 mmHg, p= ns vs. basal values). Hemorrhageinduced an increase in HR in the late phase post-hypovolemia (Hgroup: basal HR=335±6; HR 120 min=416±6*,*p<0.01 vs basalvalues). The same effect was observed in H + EPO animals,however post hemorrhagic was lower (H + EPO: basal HR=349±6; HR 120 min=380±6*#, *p<0.01 vs basal values; #p<0.01 vs H group). Conclusions: EPO pre-treatment attenuatedthe immediate hypotension and could be attenuate the tissue injury induced byacute hemorrhage. In light of the results of the present study, we suggestthat  EPO therapy modulateshemodynamic  parameters during and afteracute hemorrhage influencing the cardiovascular response  to hypovolemic  state.