IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Oxygen metabolism in the cardiorespiratory system after an acute exposure to Ni-doped nanoparticles
Autor/es:
MAGNANI, NATALIA; MARCHINI, TIMOTEO; MELE, FLAVIA; VANASCO, VIRGINIA; MEBERT, ANALÍA; DESIMONE, MARTÍN; DIAZ, LUIS; ALVAREZ, SILVIA; EVELSON, PABLO
Lugar:
Kyoto
Reunión:
Congreso; 17th Biennial Meeting of the Society for Free Radical Research International; 2014
Institución organizadora:
SFRRI
Resumen:
Transition metals such as nickel are frequent air pollution constituents and could play a central role in cardiopulmonary diseases mechanisms initiated by the exposure to ambient particulate matter (PM). The aim of this work was to analyse the cardiovascular physiopathological mechanisms observed after an acute Ni-doped nanoparticles (Ni-NP) exposure. Swiss mice were intranasally instilled with a Ni-NP suspension (0.01; 0.05; 0.1 and 1.0 mg Ni/kg body weight). Lung, heart and plasma samples were collected 1 hour after exposure. Tissue O2 consumption, NADPH oxidase (Nox) activity and TBARS content were evaluated. After the exposure to every Ni-NP dose, a significant increase in lung tissue O2 consumption was observed (60%; p˂0.0001). Nox activity (40%; p˂0.001) and TBARS content (40%; p˂0.001) in lung homogenates were also increased. TBARS content in plasma was significantly increased only in the highest assayed dose. Heart tissue cubes O2 consumption showed a 30% decrease when compared to the control group in every Ni-NP dose tested (p˂0.001). However, heart TBARS levels remained unchanged. Taken the results together, these findings provide new insights to the understanding of physiopathological mechanisms triggered by transition metals present in environmental PM.