Antihyperalgesic effect of Lithraea molleides

CATALANO ALEJANDRA, LÓPEZ PAULA,; RIEDEL R, ANZOISE L, GORZALCZANY SUSANA

Puerto Varas

Congreso; 14th International Congress of Ethnopharmacology.VIII Simposio Internacional de Química de Productos Naturales y sus Aplicaciones; 2014

International Society for Ethnopharmacology

Lithraea molleoides (Vell.) Engl. (Anacardiaceae)is a medicinal plant commonly used in traditional medicine in South America for its properties to treat inflammation of respiratory and digestive tract, as antiarthritic, diuretic and tonic. In previous investigations, L. mo/leoides showed a significant anti-inflammatory and antinociceptive activity in experimental models. So, it is expected that this species possess eseffect on hyperalgesicmodel in rats. In this sense, intraperitonealtreatment of L. mo/leoides (300 mg/kg) reduced carrageenan-induced mechanical hyperalgesia (26 % of inhibition),without any effect at dose of 100 mg/kg i.p. Mechanicalwithdrawal thresholds of the paw were evaluated using von Frey filaments. In the same model, only higher dose produced a significant inhibition of myeloperoxidase activity in rat paw, representing the activity of extract on neutrophil recruitment in paw rat. Furthermore,western blotting of paw rat revealed that 300 mg/kg i.p. of L. molleoides decreased carrageenan induced cyclooxygenase-2 expression without any effect at dose of 100 mg/kg. On the other hand, lipid oxidation products assessed by the TBARS assay in paw were significantly modified when the animals were treated with L. molleoides,showing that the extract possess antioxidant effect in vivo. These results suggest that L. molleoides aqueous extract produced antinociceptive activity in hyperalgesic model.