IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
EFFECT OF SELECTED PARAMETERS IN HYBRIDOMA EFFECT OF SELECTED PARAMETERS IN HYBRIDOMA
Autor/es:
DESIMONE, M.F.(I); M. C. DE MARZI; ALVAREZ, G.S; GANEM, B; E. L. MALCHIODI; DIAZ, L.E
Lugar:
Mar del Plata- Argentina
Reunión:
Congreso; 43th Annual Meeting - XLIII Runion Anual - SAIB; 2007
Resumen:
EFFECT OF SELECTED PARAMETERS IN HYBRIDOMA EFFECT OF SELECTED PARAMETERS IN HYBRIDOMA Desimone, M.F.; De Marzi, M.C.; Alvarez, G.S.; Ganem, B.; Malchiodi, E.L.; Diaz, L.E. Qu¨ªmica Anal¨ªtica Instrumental ¨C Inmunolog¨ªa, FFYB, UBA, IDEHU-CONICET, Buenos Aires, Argentina E-mail: desimone@ffyb.uba.ar The sol-gel process is an inorganic polymerization taking place in mild conditions, allowing the association of mineral phases with organic or biological systems. Optimization of the technological parameters affecting the mechanical properties and permeability of sol-gel matrices is essential to develop matrices with improved properties for cell immobilization. In this work, the effect of different parameters such as the concentration of different sol-gel precursors and immobilized cell density, on the function of hybridoma cells producing anti-peptidoglycan receptor protein (PGRP) I-¦Á.monoclonal antibody (mAb) has been investigated. Cell morphology was conserved as it was observed by optical and fluorescence microscopy. Cellular activity of immobilized hybridoma cells was determined by the tetrazolium assay (MTT assay) and a LIVE/DEAD cell vitality fluorescent assay. Viability was 89.5% in tetrakis (2-hydroxyethyl) ortosilitate-derived matrices and 60.5% in tetraethoxisilane- derived matrices. No differences were observed between cell densities employed in this work (5x105 ¨C 3x106 cells/ml). MAb production from immobilized cells was detected by ELISA even after 10 days post immobilization.The design of such novel devices with significant added value is a key factor when foreseeing industrial developments of sol-gel materials in medicinal science.