IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Moderate zinc deficiency during fetal and postnatal growth: effects on adult artreial blood pressure and kidney
Autor/es:
TOMAT AL; KARLÉS C; VALLONE, C; LOPEZ FERRUCCI, D; INSERRA F; BALASZCZUK AM; COSTA MA; ARRANZ C
Lugar:
South Beach, Florida
Reunión:
Congreso; Inter-American Society of Hypertension XVII Scientific Sesions; 2007
Institución organizadora:
American Society of Hypertension
Resumen:
Moderate zinc deficiency during fetal and postnatal growth: effects on adult arterial blood pressure and kidney. Tomat A, Karles C, Veiras L, Vallone C, Lopez Ferrucci D, Inserra F, Balaszczuk A, Costa A, Arranz A. Facultad de Farmacia y Bioqu¨ªmica.Universidad de Buenos Aires.IQUIMEFA-CONICET.Buenos Aires.Argentina Background:Previously,we showed that moderate zinc deficiency during post-weaning growth induces an increase in arterial blood pressure and an impaired renal function in the adult life.These alterations were associated with a decreased activity of vascular and renal nitric oxide system.Objective:We studied the effects of moderate zinc deficiency during fetal and early postnatal life on systolic blood pressure (SBP), renal function and morphology and renal oxidative stress state in the adult life. Methods:Wistar rats received from the beginning of pregnancy up to weaning low (8 ppm) or control (30 ppm) zinc diet.After weaning, male offspring of each group of mothers continued with low (Cl and Ll) or control (Cc and Lc) zinc diet during 60 days.At the end of the experimental period, we determined: SBP, creatinine clearance (Ccr), renal morphometric studies, apoptotic cells (Tunel assay), thiobarbituric acid-reactive substances (TBARS) concentration, superoxide dismutase enzyme (SOD) activity, catalase (CAT) activity in renal tissue. Results:*p<0.01 vs Cc; &p<0.01 vs Lc. SBP(mmHg): Cc:126¡À3; Cl:147¡À4 *; Lc:145¡À5*; Ll:145¡À4* Ccr(ml/min.100g):Cc:0,56¡À0.08; Cl:0,29¡À0,04*; Lc:0,32¡À0,01*; Ll: 0,26¡À0,06* Nephron number (per area):Cc:7.6¡À0.2; Cl            5.7¡À0.2*; Lc:5.6¡À0.2*; Ll:5.5¡À0.1* Total Glomerular area(¦Ìm2):Cc:19240¡À416; Cl:14600¡À478*&; Lc:16490¡À462*; Ll:14140¡À404*& Glomerular Capillary Area(¦Ìm2):Cc:13830¡À331; Cl:8671¡À358*&; Lc:11150¡À407*; Ll:7977¡À276*& Glomerular Capillary Area/Total Glomerular Area(%):Cc:72¡À1; Cl:59¡À1*&; Lc: 67¡À1*; Ll:56¡À1*& TUNEL(apoptotic cells per area):Cc:5.2¡À2.8; Cl:20.9¡À5.4*; Lc:14.1¡À4.2*; Ll:50.3¡À4.1*& TBARS(nmol/mg protein):Cc:0,16 ¡À 0,02; Cl:0,46 ¡À 0,02*&; Lc:0,38 ¡À 0,05*; Ll:0,66 ¡À 0,08*&. SOD (U/mg protein):Cc:10.3¡À1.7; Cl:11.2¡À1.5; Lc:10.7¡À1.5; Ll:12.1¡À1.1. CAT activity (pmol/mg protein): Cc:2.1¡À0.4; Cl:1.2¡À0.1*&; Lc:2.6¡À0.1; Ll: 1.4¡À0.4*&Conclusions:Our results show that lower renal filtration surface area may be responsible for the reduction of glomerular filtration rate and, probably, one of the mechanisms involved in the increase of arterial blood pressure observed in this model of moderate zinc deficiency.The activation of apoptotic mechanisms and the higher oxidative stress levels could explain, at least in part, the renal morphometric alterations. An adequate zinc intake during growth could not totally revert the effects on renal function and arterial blood pressure induced by zinc deficiency during fetal and lactation periods of life.An imbalance in zinc bioavaiability during prenatal and postnatal growing periods may be may be a risk factor in developing renal and cardiovascular alterations in adult life.