IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Zinc deficiency during intrauterine and postnatal growth induces aortic morphological and functional alterations in rats
Autor/es:
ARRANZ CRISTINA; MENDES GARRIDO FACUNDO; GOBETTO NATALIA; JURIOL LORENA; DASSO MARINA; WENK GRETEL; ELESGARAY ROSANA; CANIFFI CAROLINA; TOMAT ANALÍA
Lugar:
Atenas
Reunión:
Congreso; Meeting ESH - ISH Hypertension 2014; 2014
Institución organizadora:
European Society of Hypertension - International Society of Hypertension
Resumen:
Objetive: Moderate zinc deficiency during intrauterine and postnatal growth induces an increase in blood pressure levels in adult males related to cardiovascular and renal disorders. To evaluate aortic morphological and function in adult male rats exposed to fetal and postnatal zinc deficiency. Design and method: Female Wistar rats received during pregnacy up to weaning low (L:8 ppm) or control (C:30 ppm) zinc diet. After weaning, male off-spring fed low (l) or control (c) zinc diet during 60 days (Cc, Ll, Lc). At day 81, we measured systolic blood pressure (SBP, mmHg, tail-cuff technique) and we evaluated the thoracic aorta morphology (Sirius red staining): artery area (Aa, mm2), media area/lumen area (Ma/La, %), collagen in tunica media (Fibrosis, arbitrary score, scale 0 to 4). In aorta we measured basal nitric oxide synthase (NOS) activity, and endothelial (eNOS), neuronal (nNOS) and inducible (iNOS) isoforms activities (pmol 14C-L-citrulline/g.tissue.min). In aortic rings, suspended in Krebs solution and preconstricted with phenylephrine 10-5M, we evaluated the maximal relaxation (ACh Emax, %) with acetilcholine (10-10 to 10-3 M); we also measured the maximal contraction with angiotensine-II (Ang-II Emax, % maximal contraction with KCl 90 mM, 10-10 to 10-6 M). Values are means±SEM, n=6/group. One way ANOVA, Bonferroni post-test. Results: Cc Ll Lc SBP 126±1 143±1* 146±2* Aa 2.3±0.1 1.7±0.1*? 2.5±0.2 Ma/La 22.8±0.4 23.0±0.4 23.8±0.6 Fibrosis 1.1±0.6 3.1±0.3* 1.6±0.3 NOS activity 225±5 172±4* 160±8* Ach Emax 90±1 76±5* 77±3* Ang-II Emax 32±3 23±2* 25±2* *p