IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Age-related Aquaporin-1 changes are associated to cardiac water homeostasis during dehidration
Autor/es:
IOVANE, AGUSTINA; NETTI, VANINA; ALENCASTRO, LAURA; ZOTTA, ELSA; FELLET, ANDREA; BALASZCZUK, ANA MARÍA
Lugar:
Atenas
Reunión:
Congreso; 24th European Meeting on Hypertension; 2014
Institución organizadora:
European Society of Hypertension
Resumen:
Water channel aquaporin-1 (AQP1) is involved in the maintenance of cellular osmotic environment. Even though this protein has been linked to cardiovascular homeostasis, its physiological role still remains to be explored, particularly in the postnatal period. Objective: the aim of the present study was to evaluate cardiac AQP1 in rats subjected to hypovolemic state following water restriction during the growth stage. Methods: male Sprague-Dowley rats aged 25 and 50 days were divided in the following groups: R: water restriction during 3 days; C: water ad libitum for 3 days. At the end of each experiment, we determined: cardiac weight and water content (according to Ding et al.), cardiomyocyte mean diameter, cardiac fibrosis (Trichrome staining) and AQP1 protein levels (Western Blot) and localization (Immunohistochemistry). Results: Cardiac tissue stained with Masson's trichrome did not show increased fibrosis in neither of the experimental groups. AQP1 immunohistochemical staining of the heart revealed its presence in vascular endothelium and endocardium in control animals of both age groups. No changes in AQP1 localization or protein levels were observed in the 25-day-old group submitted to water restriction. In the oldest group, AQP1 staining appeared in the plasma membrane and its protein levels were increased in response to water restriction. Conclusion: Water restriction protocol induced a decrease in heart weight, cardiac water content and cardiomyocyte mean diameter without fibrosis development in the 25-day-old rats; such results may be compatible with microcardia. Increased AQP1 protein levels and membrane localization in the 50-day-old group may prevent such alterations, probably indicating that AQP1 participates in maintaining cardiac water homeostasis during hypovolemic state in mature rats.