Evaluation of the antiproliferative activity of an aqueous extract from L. divaricata on a murine lymphocitic leukaemia cell line: bioguided fractionation and stability study during simulated digestive process

RENZO MARTINO; VALERIA P. SULSEN; ROSARIO ALONSO; CLAUDIA ANESINI

Boston

Congreso; World Biotechnology congress 2013; 2013

EUREKA conferences

Leukemia and lymphoma are a group of heterogeneous neoplastic disorder of white blood cells. Reactive oxygen species (ROS) are involved in modulation of proliferation/death balance in cells. Conventional medicine can be inefficient or also results in side effects. Larrea divaricata Cav is a plant widely distributed in Argentina that possesses antiproliferative and antioxidant activities reported. The aim of this work was to do a bioguided fractionation of the aqueous extract (Aq) of the plant to obtain the most active fraction, for the inhibition of a murine lymphocitic leukaemia cell line (EL-4) proliferation by MTT assay, to analyze the mechanism of action in relation to ROS (spectroscopic assays), to identify the active compounds and to establish Aq stability in simulated gastric and intestinal digestive medium by HPLC. The ethyl acetate fraction (EA) was the most antiproliferative fraction (EC50: 15.4 ± 1 µg/ml), decreased cell viability inducing late apoptosis. EA increased hydrogen peroxide, and nitric oxide, decreased superoxide anion, catalase, Peroxidase and superoxide dismutase cell activities. Nordihydroguaiaretic acid and quercetin-3methyl ether were identified as majority compounds which exerted a synergistic effect. It conclusion, Aq was optimal for oral administration, suggesting that AE could be a potential therapy for lymphoma and leukemia treatment.