IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Potassium channels opening is regulated by C-type natriuretic peptide in aorta artery of spontaneously hypertensive rats
Autor/es:
COSTA MARÍA DE LOS ÁNGELES; SUEIRO LAURA; MUÑOZ FLORENCIA; WENK GRETEL; RADIONOVAS VANESA; SUÁREZ DI SALVO DAIANA; BOUCHET GONZALO; CANIFFI CAROLINA; ARRANZ CRISTINA
Lugar:
Milán
Reunión:
Congreso; 23rd European Meeting of Hypertension.; 2013
Institución organizadora:
European Society of Hypertension.
Resumen:
The aim was to study the participation of potassium channels in vascular relaxation induced by Ctype natriuretic peptide (CNP) in spontaneously hypertensive rats (SHR). Methods: Aorta thoracic rings (35 mm) were suspended in Krebs at 37ºC and gassed (95% O2 - 5% CO2, pH:7,4), allowed to equilibrate 60min) and readjusted to basal tension 1g. Isometric tension (g) was recorded by a forcetransducer, in presence of: phenylephrine (PE, 107105 M), CNP (1012106 M), LNAME (nitric oxide synthase (NOS)inhibitor, 105M), tertraethylammonium chloride (TEA, non selective K+ channel blocker, 1 mM), Cl2Ba (inward rectifier K+ channel (Kir), blocker 30 μM), glibenclamide (GLIB, ATP sensitive K+ channels blocker,KATP, 3 μM) and/or in absence of endothelium (NoE). Endothelium integrity was verified with acethylcholine (1010105 M). Results: Table: Maximal relaxation (Rmax) and concentration of the CNP (expressed as negative log molar) producing 50% of maximum relaxation (pEC50) *p