Gender differences in cardiovascular alterations induced by zinc restriction during fetal life and growth

ARRANZ CRISTINA; JURIOL LORENA VANESA; MENDES GARRIDO FACUNDO; GOBETTO NATALIA; ELESGARAY ROSANA; MAC LAUGHLIN MIRIAN; DASSO MARINA; CARDELLI ALCALDE DANIELA; ZILBERMAN JUDITH; COSTA M. ANGELES; TOMAT ANALÍA

Milan

Congreso; 23rd European Meeting on Hypertension and Cardiovascular Protection; 2013

GENDER DIFFERENCES IN CARDIOVASCULAR ALTERATIONS INDUCED BY ZINC RESTRICTION DURING FETAL LIFE AND GROWTH C. Arranz, L. Juriol, F. Mendes Garrido, N. Gobetto, R. Elesgaray, M. Mac Laughlin, M. Dasso, D. Cardelli Alcalde, J. Zilberman, M. Costa, A. Tomat. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, IQUIMEFA-CONICET, Buenos Aires, ARGENTINA Male adult rats exposed to zinc restriction in the diet during fetal life showed hypertension, renal dysfunction and alteration in lipid metabolism in the adult life. Meanwhile, 6 and 21 days-old rats showed myocytes remodelling and reduced cardiac nitric oxide synthase activity. Objective: To evaluate cardiovascular alterations in growing and adult zinc deficient rats and to compare the effects in males (m) and females (f) rats. Design and method: Wistar rats were exposed from the beginning of pregnancy up to adulthood: low (L, 8 ppm) or control(C, 30 ppm) zinc diet. At day 81, we evaluated, in m and f offspring of each group of mothers (Cm, Lm, Cf and Lf), systolic blood pressure (SBP, mmHg) by indirect tail-cuff technique, thoracic aorta diameter (AD,µm, stained by heamatoxylin-eosin), heart weight/tibial lengh (HW,mg/mm) , and left ventricular (LV) function, assessed by echocardiography, studying end "?diastolic (EDV, ml) and systolic (ESV,ml) volume and ejection fraction (EF %). Results: Zinc deficient rats showed reduced HW in both gender (Cm: 30.9±1.3, Lm: 27.3 ± 1.1*; Cf: 25.1±0.5, Lf:19.9±0.7" ). AD was reduced only in m zinc deficient rats (Cm: 1728±34; Lm:1468±41*; Cf:1551±42; Lf:1536±33, NS). Values are means±SEM, n=6 for each group.Two ways ANOVA, Bonferroni post test *p