Antiproliferative activity of the flavonoid quercetin-3-methyl-ether on a lymphoma cell line: an approach to its mechanism of action

MARTINO RENZO; SULSEN, VALERIA; ALONSO MARÍA ROSARIO; ANESINI CLAUDIA

Buenos Aires

Conferencia; VI International Conference on Polyphenols and Health ICPH-6; 2013

Facultad de Farmacia y Bioquímica UBA e Instituto de Bioquímica y Medicina Molecular UBA - Conicet

Antiproliferative activity of the flavonoid quercetin-3-methyl-ether on a lymphoma cell line: an approach to its mechanism of action Martino R., Sülsen V., Alonso MR., Anesini C. IQUIMEFA-UBA-CONICET and Pharmacognosy Unit, Faculty of Pharmacy and Biochemistry, Buenos Aires University. Junín 956 2nd floor, Ciudad Autónoma de Buenos Aires, Argentina. canesini@yahoo.com.ar. Te: 5411-49648247 Leukemia and lymphoma are a group of heterogeneous neoplastic disorder of white blood cells. Nitric oxide (NO) is involved in modulation of proliferation/death balance in cells. Conventional medicine can be inefficient or also results in side effects. Previously it was reported the antiproliferative effect of a fraction named AE ( ethyl acetate), obtained from an aqueous extract of Larrea divaricata Cav which was rich in polyphenols. One of the compounds identified was quercetin-3-methy-ether (Q-3ME). The aim of this work was to determine the effect of Q-3ME on the proliferation of the lymphoma cell line EL-4, to analyze the mechanism of action in relation to nitric oxide production and to determine the effect on cell cycle and apoptosis by flow cytometry. Q-3ME exerted antiproliferative activity (EC50: 46.7± 4 µg/ml) in relation to an arrest of cell cycle and the induction of apoptosis mediated by NO production. It conclusion, Q3-ME could be a potential therapy for lymphoma and leukemia treatments.