Early cardiovascular alterations induced by zinc deficiency during fetal life and lactation

MENDES-GARRIDO F; SANCHEZ R; VEIRAS LC; COSTA M.A; TOMAT AL; ARRANZ,C

San Diego

Congreso; Experimental Biology 2012; 2012

FASEB (Federation of American Societies for Experimental Biology) Office of Scientific Meetings and Conferences

The aim was to evaluate early cardiovascular alterations in rats exposed to moderate zinc deficiency during fetal life and lactation, and compare the effects in males (m) and females (f). Wistar rats received low (L,8 ppm) or control (C,30 ppm) zinc diet from pregnancy until offspring weaning (day 21). At 21 days, we evaluate mayor (MM) and minor (mM) myocyte diameter (μm); aortic intima (Ai), media (Am) and perivascular collagen (Ac) thickness (μm); cardiac (C) and aortic (A) nitric oxide synthase (NOS) activity (pmol Cit/g.tissue.min); cardiac thiobarbituric acid substances (Tbars, nmol/mg protein), glutathione (G, mg/g protein), glutathione peroxidase (GPX, μmol/min/mg protein), catalase (CAT, pmol/mg protein) and superoxide dismutase (SOD, U/mg protein) activity in Lm, Lf, Cm and Cf rats. Cm Lm Cf Lf MM 14±1 18±1* 15±1 15±1 mM 8,6±0,5 10,7±0,3* 9,4±0,5 10,1±0,3 Ai 23,3±0,2 18,3±0,1* 23,9±0,2 18,9±0,2? Am 280±2 298±2 287±3 280±2 Ac 16±1 24±1* 12±1 23±2? C NOS 249±5 205±5* 247±3 201±3& A NOS 225±5 172±5* 211±6 179±6& G 9±1 3±1* 10±1 6±1& GPX 81±8 65±6* 85±6 68±8& Tbars 0.41±0.05 0.32±0.02 0.22±0.04* 0.18±0.02 No differences were observed in cardiac SOD and CAT activity p