Controlled Adhesion and Proliferation of Human Osteoblastic Cells Lines by Tuning Nanoporous Titania and Silica Coatings

BELLINO MG; GOLBERT S; DE MARZI MC; SOLER ILLIA G; DESIMONE MF

Mendoza

Congreso; 48º Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2012

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Herein, we demonstrate that it is possible to tune adhesion and proliferation of human osteoblastic cell line (Saos-2) by tailoring the nanopore size of a film oxide coating. The designed strategy was based on the tailored preparation of mesoporous coatings by combination of sol-gel and supramolecular templating. Non mesoporous Si films and mesoporous Si with an average pore diameter of 4 and 9nm, as well as, non mesoporous Ti films and mesoporous Ti with an average pore diameter of 6 and 10 nm were prepared by evaporation induced self-assembly. Cellular response was dependent on both physicochemical and nano-topographical stimuli. Particularly, it was shown that in the short term (i.e.: 3-6 hours), the initial events at the surface are governed by the hydrophilic/hydrophobic properties of the surface that creates an interface to which the cell responds with different degrees of adhesion. Moreover, in the long term (days), the nanotopography and chemistry of the surface, determines how cells will attach and proliferate. In this sense, it was demonstrated that it is possible to tune cell adhesion and proliferation by controlled nanoporosity introduced during film formation. It is important to emphasize the simplicity of the procedure used to prepare these nanostructured surfaces that avoids the use of chemical functionalization.