SOL-GEL nanostructured antibiofilm glass surfaces

VILLANUEVA MARÍA EMILIA; CAMPOROTONDI DANIELA EDHIT; COPELLO GUILLERMO; TEVEZ SERGIO; DÍAZ LUIS EDUARDO

Potrero de los Funes, San Luis

Congreso; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2011

Various types of surfaces in clinical or industrial settings are prone tounwanted biofilm generation. In order to prevent the large doses ofantibiotics that are required to eradicate them, superhydrophobic surfacesare being developed as alternative. These surfaces are shown to preventcontact between a bacterium and surface attachment points and they aregenerally made from a low energy material with a highly developedmicrostructure, some incorporating two different length scales in thisroughness.In this work, we describe a simple method to fabricate superhydrophobicsol-gel nanostructured glass coatings to control bacterial adhesion. Thesesurfaces were manufactured by coating glass slides with a mixture ofAminopropyl trietoxysilane and Tetraetoxysilane in order to functionalizethe surface with amino groups. Then, two different sizes of silicaparticles: nanoparticles (NPs: 27 nm) and microparticles (MPs: 2 μm)were also functionalized with NH2 groups and attached to the surface byglutarahaldeyde crosslinking. The effect of different proportions of theparticles was studied (0:100, 25:75, 50:50 Mps:NPs). Finally, the surfaceswere treated with dodecenyl succinic anhydride in anhydrous ethanol (1/10,1/100, 1/1000). Surfaces hydrophilic/hydrophobic behavior was analyzed bymeans of contact angle and inhibition of biofilm formation by exposing thesurfaces to Pseudomonas spp.