IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cellularized Silica-Collagen Bionanocomposites
Autor/es:
S. QUIGNARD (SP); M.F. DESIMONE; C. HÉLARY; M.M. GIRAUD-GUILLE; T. CORADIN
Lugar:
Jena
Reunión:
Simposio; Euromat 2011. European Symposium on Biomaterials and related Areas; 2011
Resumen:
Cellularized collagen hydrogels are commonly obtained using the method developed by Bell et al., consisting in the neutralization of diluted acid soluble collagen solutions extemporary prior to fibroblast addition.1 One of the main limitations in using those types of collagen gels as biomedical materials is related to their strong contraction upon ageing. With the aim of reducing this contraction effect, we have prepared silica-collagen composite hydrogels, relying on the addition of small amounts of silica colloids to cold acidified collagen solutions before neutralization.2,3 While studying these materials, silica particles were found to diffuse in the gel, to be internalized by immobilized cells and to be released from the gel. To get a better understanding of these processes, the diffusion of silica nanoparticles in acellularized and cellularized collagen hydrogels was studied. The effect of different parameters on the diffusion, dissolution and internalization of silica nanoparticles were investigated, including the size of the particles (in the range of 10nm-200nm), their initial surface charge, the influence of cells and the concentration of collagen. In parallel, cell viability was estimated by MTT tests. The results show that silica nanoparticles efficiently slow down collagen hydrogel contraction while preserving fibroblast viability. In addition, silica particles are (i) interacting with collagen as a function of their size and surface charge, (ii) influencing fibroblast behavior by being uptaken by immobilized cells, (iii) diffusing through the gel and being released with kinetics that are size- and charge-dependent and (iv) partly dissolving, releasing any entrapped species.