IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Critical role of endogenous galectin-1 in regulating T helper responses during Yersinia enterocolitica infection
Autor/es:
DAVICINO, ROBERTO ; ELIÇABE, JAVIER; DI GENARO, SILVIA; RABINOVICH, GABRIEL A
Lugar:
Buenos Aires
Reunión:
Congreso; LVIII Reunión Sociedad Argentina de Inmunología; 2010
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
In spite of considerable advances in elucidating the immunomodulatory
functions of galectin-1 in limiting Th1 and Th17 responses
in autoimmune settings, the role of this endogenous
lectin in microbial infection has not yet been examined. Given
that Yersinia enterocolitica, Gram-negative bacteria evokes Th1-
producing IFN-g immune response, we conducted this study to
investigate the absence of endogenous galectin-1 in the development
of immunity against this pathogen. We used C57BL/6
wild-type (WT) and Gal-1 knockout (Lgals1-/-) mice which were
orogastrically infected with Y. enterocolitica O:8. Survival was
compared in both infected mouse groups. On days 5, 14 and 21
after infection, mesenteric lymph nodes (MLN), spleen (Sp) and
Peyers patches (PP) were aseptically obtained, and in their homogenates,
colony forming units (CFU) were counted. In addition,
we determined concentrations of IL-17 and IFN-g in PP and
serum by ELISA. To elucidate their immunoregulatory effects,
spleen cells from Lgals1-/- mice were adoptively transferred
into WT mice; then, these recipient mice were challenged with
Y. enterocolitica and CFU in organs, IL-17 and IFN-? in PP and
serum at 5 days after infection were determined. Higher survival
rate was observed in Lgals1-/- compared WT mice (63 % vs 37
%). Furthermore, less CFU in PP after 5 and 14 d after infection
of Lgals1-/- mice (p <0.05) and adoptived-transferred WT mice
(p<0.05) were determined. At day 21, we did not find CFU in
PP, though, we found a decreased counting of CFU on Sp of
Lgals1-/- mice (p<0.05). Moreover, higher levels of IFN-g and IL-
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.
serum by ELISA. To elucidate their immunoregulatory effects,
spleen cells from Lgals1-/- mice were adoptively transferred
into WT mice; then, these recipient mice were challenged with
Y. enterocolitica and CFU in organs, IL-17 and IFN-? in PP and
serum at 5 days after infection were determined. Higher survival
rate was observed in Lgals1-/- compared WT mice (63 % vs 37
%). Furthermore, less CFU in PP after 5 and 14 d after infection
of Lgals1-/- mice (p <0.05) and adoptived-transferred WT mice
(p<0.05) were determined. At day 21, we did not find CFU in
PP, though, we found a decreased counting of CFU on Sp of
Lgals1-/- mice (p<0.05). Moreover, higher levels of IFN-g and IL-
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.
investigate the absence of endogenous galectin-1 in the development
of immunity against this pathogen. We used C57BL/6
wild-type (WT) and Gal-1 knockout (Lgals1-/-) mice which were
orogastrically infected with Y. enterocolitica O:8. Survival was
compared in both infected mouse groups. On days 5, 14 and 21
after infection, mesenteric lymph nodes (MLN), spleen (Sp) and
Peyers patches (PP) were aseptically obtained, and in their homogenates,
colony forming units (CFU) were counted. In addition,
we determined concentrations of IL-17 and IFN-g in PP and
serum by ELISA. To elucidate their immunoregulatory effects,
spleen cells from Lgals1-/- mice were adoptively transferred
into WT mice; then, these recipient mice were challenged with
Y. enterocolitica and CFU in organs, IL-17 and IFN-? in PP and
serum at 5 days after infection were determined. Higher survival
rate was observed in Lgals1-/- compared WT mice (63 % vs 37
%). Furthermore, less CFU in PP after 5 and 14 d after infection
of Lgals1-/- mice (p <0.05) and adoptived-transferred WT mice
(p<0.05) were determined. At day 21, we did not find CFU in
PP, though, we found a decreased counting of CFU on Sp of
Lgals1-/- mice (p<0.05). Moreover, higher levels of IFN-g and IL-
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.
serum by ELISA. To elucidate their immunoregulatory effects,
spleen cells from Lgals1-/- mice were adoptively transferred
into WT mice; then, these recipient mice were challenged with
Y. enterocolitica and CFU in organs, IL-17 and IFN-? in PP and
serum at 5 days after infection were determined. Higher survival
rate was observed in Lgals1-/- compared WT mice (63 % vs 37
%). Furthermore, less CFU in PP after 5 and 14 d after infection
of Lgals1-/- mice (p <0.05) and adoptived-transferred WT mice
(p<0.05) were determined. At day 21, we did not find CFU in
PP, though, we found a decreased counting of CFU on Sp of
Lgals1-/- mice (p<0.05). Moreover, higher levels of IFN-g and IL-
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.
g immune response, we conducted this study to
investigate the absence of endogenous galectin-1 in the development
of immunity against this pathogen. We used C57BL/6
wild-type (WT) and Gal-1 knockout (Lgals1-/-) mice which were
orogastrically infected with Y. enterocolitica O:8. Survival was
compared in both infected mouse groups. On days 5, 14 and 21
after infection, mesenteric lymph nodes (MLN), spleen (Sp) and
Peyers patches (PP) were aseptically obtained, and in their homogenates,
colony forming units (CFU) were counted. In addition,
we determined concentrations of IL-17 and IFN-g in PP and
serum by ELISA. To elucidate their immunoregulatory effects,
spleen cells from Lgals1-/- mice were adoptively transferred
into WT mice; then, these recipient mice were challenged with
Y. enterocolitica and CFU in organs, IL-17 and IFN-? in PP and
serum at 5 days after infection were determined. Higher survival
rate was observed in Lgals1-/- compared WT mice (63 % vs 37
%). Furthermore, less CFU in PP after 5 and 14 d after infection
of Lgals1-/- mice (p <0.05) and adoptived-transferred WT mice
(p<0.05) were determined. At day 21, we did not find CFU in
PP, though, we found a decreased counting of CFU on Sp of
Lgals1-/- mice (p<0.05). Moreover, higher levels of IFN-g and IL-
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.
serum by ELISA. To elucidate their immunoregulatory effects,
spleen cells from Lgals1-/- mice were adoptively transferred
into WT mice; then, these recipient mice were challenged with
Y. enterocolitica and CFU in organs, IL-17 and IFN-? in PP and
serum at 5 days after infection were determined. Higher survival
rate was observed in Lgals1-/- compared WT mice (63 % vs 37
%). Furthermore, less CFU in PP after 5 and 14 d after infection
of Lgals1-/- mice (p <0.05) and adoptived-transferred WT mice
(p<0.05) were determined. At day 21, we did not find CFU in
PP, though, we found a decreased counting of CFU on Sp of
Lgals1-/- mice (p<0.05). Moreover, higher levels of IFN-g and IL-
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.
g in PP and
serum by ELISA. To elucidate their immunoregulatory effects,
spleen cells from Lgals1-/- mice were adoptively transferred
into WT mice; then, these recipient mice were challenged with
Y. enterocolitica and CFU in organs, IL-17 and IFN-? in PP and
serum at 5 days after infection were determined. Higher survival
rate was observed in Lgals1-/- compared WT mice (63 % vs 37
%). Furthermore, less CFU in PP after 5 and 14 d after infection
of Lgals1-/- mice (p <0.05) and adoptived-transferred WT mice
(p<0.05) were determined. At day 21, we did not find CFU in
PP, though, we found a decreased counting of CFU on Sp of
Lgals1-/- mice (p<0.05). Moreover, higher levels of IFN-g and IL-
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.
g and IL-
17 were detected in sera collected from Lgals1-/- mice and also
from adoptive-transferred WT mice (p<0.05). We conclude that
the absence of Gal-1 enhances Th1 and Th17 responses which
can act to maintain protective immune response favouring
eradication of Y. enterocolitica. This is the first report demonstrating
a role of endogenous galectin-1 in regulating immunity
to infection.