IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of a fraction obtained from Larrea divaricata on dectin-1 receptor in Candida albicans infection model.
Autor/es:
MARTINO R; DAVICINO R; MATTAR A, ; CASALI Y ; MICALIZZI B
Lugar:
Ciudad de la Punta, San Luis
Reunión:
Congreso; XXVIII Reunión Anual Científica de la Sociedad de Biología de Cuyo; 2010
Institución organizadora:
Socieda dde Biología de Cuyo
Resumen:
Larrea divaricata is a shrub with several applications in argentinean
folk medicine. The aim of this work was to test the role of â-glucan
receptor (dectin-1) on macrophage (MO) of mice infected with C.
albicans and treated with a fraction obtained from L. divaricata
albicans and treated with a fraction obtained from L. divaricata
receptor (dectin-1) on macrophage (MO) of mice infected with C.
albicans and treated with a fraction obtained from L. divaricata
albicans and treated with a fraction obtained from L. divaricata
folk medicine. The aim of this work was to test the role of â-glucan
receptor (dectin-1) on macrophage (MO) of mice infected with C.
albicans and treated with a fraction obtained from L. divaricata
albicans and treated with a fraction obtained from L. divaricata
receptor (dectin-1) on macrophage (MO) of mice infected with C.
albicans and treated with a fraction obtained from L. divaricata
albicans and treated with a fraction obtained from L. divaricata
is a shrub with several applications in argentinean
folk medicine. The aim of this work was to test the role of â-glucan
receptor (dectin-1) on macrophage (MO) of mice infected with C.
albicans and treated with a fraction obtained from L. divaricata
albicans and treated with a fraction obtained from L. divaricata
receptor (dectin-1) on macrophage (MO) of mice infected with C.
albicans and treated with a fraction obtained from L. divaricata
albicans and treated with a fraction obtained from L. divaricata
â-glucan
receptor (dectin-1) on macrophage (MO) of mice infected with C.
albicans and treated with a fraction obtained from L. divaricata
albicans and treated with a fraction obtained from L. divaricata
C.
albicans and treated with a fraction obtained from L. divaricataand treated with a fraction obtained from L. divaricata
(F1). Healthy and infected mice were treated with: a) PBS; b) F1;
c) antibody against dectin-1(15Y9); d) F1+15Y9. The 15Y9 was
administered during 4 days before infection. Treatment with F1
was started 24 h after infection and extended during 3 days. MO
were harvested and, liver, spleen and kidney were removed and
homogenated for CFU counts. Phagocytosis activity, superoxide
production by NBT test, viability by MTT test and CR3 expression
on MO were performed. Results show that 15Y9 increases phagocytosis
in all groups (p<0.05). A slight decrease on phagocytosis
and NBT test was observed compared to controls (p<0.05) when
MO were treated with F1+15Y9. F1 increases viability (p<0.01),
but when dectin-1 is blocked, this viability decreases. MO from
mice treated with F1 increases the expression of CR3, but the highest
expression was observed on infected mice treated with F1+15Y9.
CFU count shows that mice treated with F1+15Y9 were able to
resolve the infection. In conclusion, F1 could act mainly through
dectin-1, and secondarily through other receptors such as CR3 to
eliminate infection.