Effect of cardiac renin angiotensin system axis on male and female rats exposed to moderate zinc restriction during fetal and postnatal life.

GOBETTO, MARÍA NATALIA; MEDINA, AGUSTINA; ELESGARAY, ROSANA; MENDES GARRIDO, FACUNDO; GIRONACCI, MARIELA; ARRANZ, CRISTINA; PÁEZ, DIAMELA; GIACOMAZI, JULIA; CANIFFI, CAROLINA; TOMAT, ANALÍA

CABA

Congreso; Reunión conjunta SAIC-SAI-AAFE-NANOMED; 2021

SAIC-SAI-AAFE-NANOMED

Introduction: We have demonstrated that zinc deficiency during pregnancy and postnatal life induced cardiovascular alterations in males. Females did not show significant heart alterations.Objective: Evaluate cardiac renin angiotensin system in adult rats exposed to zinc deficiency during growth. Methods: Female Wistar rats fed either control (C30ppm) or low zinc (L8ppm) diets from pregnancy to offspring weaning. C male and female offspring continued with control diet (Ccm;Ccf respectively), whereas L male and female offspring fed control (Lcm;Lcf) or low zinc (Llm;Llh) diets. At day 81 we measured in left ventricle: Angiotensin (Ang) II, Ang(1-7) levels by radioimmunoassay, Angiotensin converting enzyme 1(ECA1),ECA2 and AT1 receptor (AT1R) expression by RT-qPCR; AT2R and AT1R expression by western blot and immunohistochemistry. Values are means±SEM, Two-way ANOVA, Bonferroni post-test. *p