IQUIMEFA 05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
artículos
Título:
Effect of caffeine on the secretion of peroxidase in rat submandibular gland: a study of its mechanism of action.
Autor/es:
SEBASTIAN TURNER; MARIA GABRIELA MANUELE; ROBERTO DAVICINO; GRACIELA FERRARO; ROSANA FILIP; CLAUDIA ANESINI
Revista:
ARCHIVES OF ORAL BIOLOGY
Editorial:
Elsevier
Referencias:
Año: 2009 vol. 54 p. 179 - 184
ISSN:
0003-9969
Resumen:
Objetive: In this work, we analysed the mechanism of action of caffeine on peroxidase secretion in the female rat submandibular gland. The signaling molecules cAMP and nitric oxide were monitored as potential mediators. secretion in the female rat submandibular gland. The signaling molecules cAMP and nitric oxide were monitored as potential mediators. secretion in the female rat submandibular gland. The signaling molecules cAMP and nitric oxide were monitored as potential mediators. In this work, we analysed the mechanism of action of caffeine on peroxidase secretion in the female rat submandibular gland. The signaling molecules cAMP and nitric oxide were monitored as potential mediators. Design: The salivary gland peroxidase secretion of female albino Wistar rats was assessed by a spectroscopic method. a spectroscopic method. a spectroscopic method. The salivary gland peroxidase secretion of female albino Wistar rats was assessed by a spectroscopic method. Results: Caffeine was found to exert an increase on peroxidase secretion in a concentrationresponse manner: the peroxidase secretion stimulation index (SI) (secreted peroxidase from treated/secreted peroxidase from basal) for caffeine 10 mg/ml: 2.2 0.18 (P < 0.05); caffeine 100 mg/ml alone: 3 0.18 (P < 0.01); +LNMMA (LN monomethyl arginine): 1 0.1 (P < 0.05); caffeine 1000 mg/ml alone: 5 0.35 (P < 0.01); +LNMMA: 2 0.2 (P < 0.05). These results were associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). caffeine 1000 mg/ml alone: 5 0.35 (P < 0.01); +LNMMA: 2 0.2 (P < 0.05). These results were associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). caffeine 1000 mg/ml alone: 5 0.35 (P < 0.01); +LNMMA: 2 0.2 (P < 0.05). These results were associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 100 mg/ml alone: 3 0.18 (P < 0.01); +LNMMA (LN monomethyl arginine): 1 0.1 (P < 0.05); caffeine 1000 mg/ml alone: 5 0.35 (P < 0.01); +LNMMA: 2 0.2 (P < 0.05). These results were associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). caffeine 1000 mg/ml alone: 5 0.35 (P < 0.01); +LNMMA: 2 0.2 (P < 0.05). These results were associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). caffeine 1000 mg/ml alone: 5 0.35 (P < 0.01); +LNMMA: 2 0.2 (P < 0.05). These results were associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 mg/ml alone: 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 4.8 0.3 (P < 0.01); +LNMMA: 2.3 0.18 (P < 0.05). 100 mg/ml alone: 3 0.18 (P < 0.01); +LNMMA (LN monomethyl arginine): 1 0.1 (P < 0.05); caffeine 1000 mg/ml alone: 5 0.35 (P < 0.01); +LNMMA: 2 0.2 (P < 0.05). These results were associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 mg/ml alone: 2.8 0.2 (P < 0.01); +LNMMA: 1 0.08 (P < 0.05); caffeine 1000 m
