ONE-POT PREPARATION OF SBP-PANI-PAA-ETHYLENE GLYCOL DIGLYCIDYLETHER SENSOR FOR ELECTROCHEMICAL DETECTION OF H2O2

DIMAS IGNACIO TORRES; DIMAS IGNACIO TORRES; V. CAMPO DALL' ORTO; V. CAMPO DALL' ORTO; M. VICTORIA MIRANDA; M. VICTORIA MIRANDA

SENSORS AND ACTUATORS B-CHEMICAL

ELSEVIER SCIENCE SA

Lugar: Amsterdam; Año: 2017 vol. 239 p. 1016 - 1025

0925-4005

tSoybean seed coat peroxidase (SBP) was used in combination with aniline (ANI), acrylic acid (AA), ethy-lene glycol diglycidyl ether (EGDE) and H2O2, to prepare a biosensor in one-pot synthesis strategy. SBPcatalyzed the chemical polymerization of ANI initiated by H2O2, to give the conductive polymer (PANI)in acidic medium where acrylate acted as dopant. Further reactions between free radicals, AA and thediepoxy EGDE determined the entrapment of the SBP together with PANI in a hydrogel on the electrodesurface, becoming wired for the amperometric detection of H2O2at neutral pH and −100 mV vs Ag/AgCl.On the other hand, the absence of AA and EGDE in the reaction mixture led to an unstable sensor modifierand an erratic response to H2O2. The apparent Kmfor immobilized SBP in the flow system was 0.0763 mM,lower than the parameter for the free enzyme at pH 7.0. Quantitative analysis of H2O2was carried outin a flow injection system (FIA); the calibration plot was linear in the range of 5.0?50 M, with a limitof detection of 2.2 M. A dental bleaching sample was successfully analyzed and the catalase test resultsdemonstrated the specificity of the response.