IQUIMEFA 05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
artículos
Título:
The sesquiterpene lactone polymatin B from Smallanthus sonchifolius induces different cell death mechanisms in three cancer cell lines
Autor/es:
DE FORD CHRISTIAN; ULLOA, JERÓNIMO; CATALÁN CÉSAR; GRAU ALFREDO; MARTINO, VIRGINIA; MUSCHIETTI, LILIANA; MERFORT IRMGARD
Revista:
PHYTOCHEMISTRY
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2015 vol. 117 p. 332 - 339
ISSN:
0031-9422
Resumen:
A 8b-angeloyloxy-9a-hydroxy-14-oxo-acanthospermolide and five known melampolide sesquiterpenelactones (uvedalin, enhydrin, polymatin B, sonchifolin, and fluctuanin) were isolated from the leaves ofSmallanthus sonchifolius. The compounds were identified by 1D-, 2D-NMR, HRMS, IR and UV analyses.In vitro cytotoxicity assays (MTT) showed that these sesquiterpene lactones display poor cytotoxic effectson peripheral blood mononuclear cells (PBMC) of healthy human subjects, whereas a strong cytotoxicitywas observed in leukemia and pancreas cancer cells. For the mechanism of action of polymatin B,oxidative stress seems to be involved. Interestingly, reactive oxygen species (ROS) formation mainlyinduced different effects: apoptosis in CCRF-CEM cells, necroptosis in CEM-ADR5000 cells throughinduction of RIP1K, neither apoptosis nor necroptosis in MIA-PaCa-2 cells. Additionally, cells also diedpartly by necrosis.
