Cross-reaction between proteins of Larrea divaricata Cav. (jarilla) and proteins of Gram-negative bacteria.

MARÍA AÍDA MATTAR DE ANAYA; ROBERTO DAVICINO; YOLANDA CASALI; SILVIA CORREA; BLAS MICALIZZI

IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY

Taylor & Francis Group

Lugar: Londres; Año: 2009

0892-3973

Larrea divaricata is an abundant plant of the Northwest of Argentine employed to treat different pathologies. We aimed to characterize the immunogenicity of proteins from a partially purified crude aqueous extract (JPCE) of jarilla. We evaluated the cross reaction between JPCE and whole cell-bacterial proteins (W-CBP) of Escherichia coli, Pseudomonas aeruginosa, Proteus vulgarisis an abundant plant of the Northwest of Argentine employed to treat different pathologies. We aimed to characterize the immunogenicity of proteins from a partially purified crude aqueous extract (JPCE) of jarilla. We evaluated the cross reaction between JPCE and whole cell-bacterial proteins (W-CBP) of Escherichia coli, Pseudomonas aeruginosa, Proteus vulgarisEscherichia coli, Pseudomonas aeruginosa, Proteus vulgaris and Klebsiella pneumoniae using a mouse anti-JPCE serum. Protein profiles of JPCE and WCBP were analysed. For JPCE, 18 bands were observed in a 20-176 kDa range. Levels of IgG against JPCE and W-CBP were determined. Bacterial proteins showed a strong reaction with the anti-JPCE serum. Plant proteins could be used as immune stimulants.Klebsiella pneumoniae using a mouse anti-JPCE serum. Protein profiles of JPCE and WCBP were analysed. For JPCE, 18 bands were observed in a 20-176 kDa range. Levels of IgG against JPCE and W-CBP were determined. Bacterial proteins showed a strong reaction with the anti-JPCE serum. Plant proteins could be used as immune stimulants.