Influence of silicification on the structural and biological properties of buffer-mediated collagen hydrogels

S. QUIGNARD; G. J. COPELLO; C. AIME; I. BATAILLE; C. HELARY; M. F. DESIMONE; T. CORADIN

ADVANCED ENGINEERING MATERIALS (PRINT)

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2012 vol. 14 p. 51 - 55

1438-1656

A buffer-mediated gelation route for collagen hydrogels that allows the formation ofhomogeneous composite and hybrid materials with various silica sources (i.e. colloidal silicaand soluble silicates) at high concentration (up to 25 mM) is described. Most significantimprovement in rheological properties and proliferation of primary adult human dermalfibroblasts was obtained for the silicate-based hybrid materials. A similar trend was observedin composite materials incorporating 14 nm SiO2 nanoparticles, although to a much lesserextent, whereas larger colloids (80 nm and 390 nm) did not significantly impact mechanicalstability and cell behavior. Modification of 80 nm particles surface with amine groupsweakens the collagen-mineral interface, resulting in the decrease of material stability andleading to particle aggregation during the course of cell proliferation experiments.