CONICET | Buscador de Institutos y Recursos Humanos

a b s t r a c t Aim of the study: In this study the anti-inflammatory activity of aqueous, dichloromethane (CH2Cl2) and methanolic (MeOH) extracts and two major compounds isolated from Lithrea molleoides (Vell.) Engl. (Anacardiaceae) were evaluated. (Anacardiaceae) were evaluated. methanolic (MeOH) extracts and two major compounds isolated from Lithrea molleoides (Vell.) Engl. (Anacardiaceae) were evaluated. (Anacardiaceae) were evaluated. In this study the anti-inflammatory activity of aqueous, dichloromethane (CH2Cl2) and methanolic (MeOH) extracts and two major compounds isolated from Lithrea molleoides (Vell.) Engl. (Anacardiaceae) were evaluated. (Anacardiaceae) were evaluated. Lithrea molleoides (Vell.) Engl. (Anacardiaceae) were evaluated. Materials and methods: Two classical experimental models were used, carrageenan-induced rat paw edema and 12-O-tetradecanoylphorbol-13 acetate (TPA) induced mouse ear edema. edema and 12-O-tetradecanoylphorbol-13 acetate (TPA) induced mouse ear edema. Two classical experimental models were used, carrageenan-induced rat paw edema and 12-O-tetradecanoylphorbol-13 acetate (TPA) induced mouse ear edema. Results: MeOH extracts exhibited a significant systemical anti-inflammatory effect in the carrageenan (inhibition of 46% at 3 h) and in the TPA-ear edema test (inhibition of 21%). The presence of methyl gallate (inhibition of 63% in TPA ear edema), as one of the main compounds in the active fraction from MeOH extract may be explained the effect observed. Also, 1,3-dihydroxy-(Z,Z)-5-(tridec-4,7ˇıdienyl) benzene obtained from CH2Cl2 extract showed a significant topical anti-inflammatory activity (inhibition of 68%). Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. obtained from CH2Cl2 extract showed a significant topical anti-inflammatory activity (inhibition of 68%). Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. (inhibition of 46% at 3 h) and in the TPA-ear edema test (inhibition of 21%). The presence of methyl gallate (inhibition of 63% in TPA ear edema), as one of the main compounds in the active fraction from MeOH extract may be explained the effect observed. Also, 1,3-dihydroxy-(Z,Z)-5-(tridec-4,7ˇıdienyl) benzene obtained from CH2Cl2 extract showed a significant topical anti-inflammatory activity (inhibition of 68%). Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. obtained from CH2Cl2 extract showed a significant topical anti-inflammatory activity (inhibition of 68%). Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. MeOH extracts exhibited a significant systemical anti-inflammatory effect in the carrageenan (inhibition of 46% at 3 h) and in the TPA-ear edema test (inhibition of 21%). The presence of methyl gallate (inhibition of 63% in TPA ear edema), as one of the main compounds in the active fraction from MeOH extract may be explained the effect observed. Also, 1,3-dihydroxy-(Z,Z)-5-(tridec-4,7ˇıdienyl) benzene obtained from CH2Cl2 extract showed a significant topical anti-inflammatory activity (inhibition of 68%). Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. obtained from CH2Cl2 extract showed a significant topical anti-inflammatory activity (inhibition of 68%). Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. ,7ˇıdienyl) benzene obtained from CH2Cl2 extract showed a significant topical anti-inflammatory activity (inhibition of 68%). Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. 2Cl2 extract showed a significant topical anti-inflammatory activity (inhibition of 68%). Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. Conclusions: The results of this study present evidence that Lithrea molleoides given either systemically or topically has anti-inflammatory properties. or topically has anti-inflammatory properties. The results of this study present evidence that Lithrea molleoides given either systemically or topically has anti-inflammatory properties. © 2010 Elsevier Ireland Ltd. All rights reserved.