In vitro antiplasmodial activity of sesquiterpene lactones from Ambrosia tenuifolia

SÜLSEN V; GUTIERREZ YAPU D; LAURELLA L; ANESINI C; GIMENEZ TURBA A; MARTINO V; MUSCHIETTI L

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE

OXFORD UNIV PRESS

Lugar: New York; Año: 2011 p. 1 - 4

1741-427X

The in vitro antiplasmodial activity of Ambrosia tenuifolia organic extract and its isolated sesquiterpene lactones, psilostachyin and peruvin, has been evaluated against Plasmodium falciparum F32 and W2 strains. The cytotoxicity of both compounds was determined on lymphoid cells, and their corresponding selectivity indexes (SIs) were calculated. Peruvin was the most active compound on F32 strain of P. falciparum with a 50% inhibitory concentration value (IC50) of 0.3ìg/mL (1.1 ìM) whereas psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively determined on lymphoid cells, and their corresponding selectivity indexes (SIs) were calculated. Peruvin was the most active compound on F32 strain of P. falciparum with a 50% inhibitory concentration value (IC50) of 0.3ìg/mL (1.1 ìM) whereas psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively and peruvin, has been evaluated against Plasmodium falciparum F32 and W2 strains. The cytotoxicity of both compounds was determined on lymphoid cells, and their corresponding selectivity indexes (SIs) were calculated. Peruvin was the most active compound on F32 strain of P. falciparum with a 50% inhibitory concentration value (IC50) of 0.3ìg/mL (1.1 ìM) whereas psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively determined on lymphoid cells, and their corresponding selectivity indexes (SIs) were calculated. Peruvin was the most active compound on F32 strain of P. falciparum with a 50% inhibitory concentration value (IC50) of 0.3ìg/mL (1.1 ìM) whereas psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively in vitro antiplasmodial activity of Ambrosia tenuifolia organic extract and its isolated sesquiterpene lactones, psilostachyin and peruvin, has been evaluated against Plasmodium falciparum F32 and W2 strains. The cytotoxicity of both compounds was determined on lymphoid cells, and their corresponding selectivity indexes (SIs) were calculated. Peruvin was the most active compound on F32 strain of P. falciparum with a 50% inhibitory concentration value (IC50) of 0.3ìg/mL (1.1 ìM) whereas psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively determined on lymphoid cells, and their corresponding selectivity indexes (SIs) were calculated. Peruvin was the most active compound on F32 strain of P. falciparum with a 50% inhibitory concentration value (IC50) of 0.3ìg/mL (1.1 ìM) whereas psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively Plasmodium falciparum F32 and W2 strains. The cytotoxicity of both compounds was determined on lymphoid cells, and their corresponding selectivity indexes (SIs) were calculated. Peruvin was the most active compound on F32 strain of P. falciparum with a 50% inhibitory concentration value (IC50) of 0.3ìg/mL (1.1 ìM) whereas psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively P. falciparum with a 50% inhibitory concentration value (IC50) of 0.3ìg/mL (1.1 ìM) whereas psilostachyin showed activity on both strains (IC50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively 50 = 0.6 (2.1 ìM) and 1.8 ìg/mL (6.4 ìM)). Fifty percent cytotoxic concentration (CC50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively50) values (48 h) were 6.8 ìg/mL (24.3 ìM) and 10.0 ìg/mL (37.9 ìM) for psilostachyin and peruvin, respectively