“In vivo” murine macrophages activation by a dichloromethane extract of Tilia x viridis

ROBERTO DAVICINO; PATRICIA MICUCCI; GABRIELA ZETTLER; GRACIELA FERRARO; CLAUDIA ANESINI

IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY

TAYLOR & FRANCIS INC

Lugar: Londres; Año: 2010 vol. 32 p. 473 - 480

0892-3973

Macrophages are involved in the host defense against infectious pathogens and tumors. Tilia species havebeen used in folk medicine for the treatment of infectious diseases, previously it was demonstrated that adichloromethane (DM) extract possess antiproliferative action “in vitro” on a lymphoma cell line. The aim ofthis work was to study the “in vivo” effect of DM extract upon mice peritoneal macrophages. DM extract–activated macrophages phagocytosis through hydrogen peroxide (H2O2) and nitric oxide (NO) production(phagocytosis (%): basal 16.93 ± 0.18, DM extract 25.93 ± 2.8; H2O2 (M): basal 0.0022 ± 0.00016, DM extract0.0036 ± 0.0005; NO (mM): basal 0.0052 ± 0.0007, DM extract 0.0099 ± 0.0004). These actions were mediatedby cell superoxide dismutase activation. On the other hand, DM extract decreased tumor necrosis factorα but increased interleukin-10 in serum. These results suggest that the modulation activity exerted by theextract on immune system cells could be an important mechanism to acquire resistance to tumors andinfectious diseases.