EVALUATION BY LC/MS AND PRINCIPAL COMPONENT ANALYSIS OF FUNGAL CULTURES WITH HISTONE DEACETYLASE INHIBITORS AS EPIGENETIC MODIFIERS

M. ALEJANDRA RODRIGUEZ; GASTÓN E. SILESS; CABRERA GABRIELA M.; ALICIA M. GODEAS

Rosario

Simposio; Second Latin American Metabolic Profiling Symposium; 2016

LAMPS

Mass spectrometry (MS) has become one of the most valuable tools on natural products research, allowing complex mixture analysis by hyphenation with chromatographic techniques such as liquid chromatography (LC/MS)1. The study of fungal metabolome is still a challenging task, these organisms often yield compounds of unprecedented structures, which requires intensive structure elucidation by MS and NMR. However, as metabolite production of fungal cultures are dependent on environment conditions, different culture methods2 and dereplication3 techniques has been developed. The use of suberoylanilide hydroxamic acid (SAHA) and other histone deacetilase inhibitors as epigenetic modifiers was evaluated in order to observe changes in the metabolite production of the dark septate fungal endophyte Drechslera sp., isolated from the roots of rye grass (Lollium sp.). LC/MS and LC/MS/MS were employed for the analysis of the cultures. Unsupervised and supervised principal component analysis of LC/MS data were performed using two different software packages, Bruker Profile Analysis 2.0 and MZmine 2.21, which differ mainly on data treatment prior to PCA itself. Results on both platforms were compared. Several differences in the metabolite production were detected. The release of hexosyl phytosphyngosine(HPS) to the culture medium was observed using SAHA as an additive of the cultures, figure 1. The biotransformation of the inhibitors was observed in addition to the production of antifungal metabolites, showing the ability of this endophytic strain to control xenobiotics.