Study by Electrospray Ionization Mass Spectrometry of the complexation of histone deacetilase inhibitors with divalent metal ions

GASTÓN SILESS; GABRIELA M CABRERA

Conferencia; 20th International Mass Spectrometry Conference; 2014

International Mass Spectrometry Foundation

Histone deacetylase inhibitors (HDACIs) are a class of anti-cancer drugs which act as epigenetic modifiers. The HDAC family of enzymes consists of 18 known members divided into four classes, three of which are metalloenzymes that require a divalent metal ion for substrate binding and catalysis. Different classes of HDACIs are known, some of which are hydroxamic acids that chelate active Zn2+ in a bidentate manner. Since the mode of action of these compounds is related to its binding capacity of Zn2+, and they are known to chelate other metals such as Co, Cu or Ni, the goal of this study was the analysis by Electrospray of the complexes obtained from HDACIs in the presence of Zn2+ and other metals and compare their differences.