Cyclosporine A from a nonpathogenic Fusarium oxysporum suppressing Sclerotinia sclerotiorum

RODRIGUEZ, MARÍA ALEJANDRA; GABRIELA MYRIAM CABRERA; GODEAS, ALICIA

Journal of Applied Microbiology

Año: 2006 vol. 100 p. 575 - 586

1364-5072

Aims: To evaluate the antagonistic activity of Fusarium oxysporum nonpathogenic fungal strain S6 against the phytopathogenic fungus Sclerotinia sclerotiorum fungal strain S6 against the phytopathogenic fungus Sclerotinia sclerotiorum To evaluate the antagonistic activity of Fusarium oxysporum nonpathogenic fungal strain S6 against the phytopathogenic fungus Sclerotinia sclerotiorumSclerotinia sclerotiorum and to identify the antifungal compounds involved. Methods and Results: The antagonistic activity of Fusarium oxysporum strain S6 was determined in vitro by dual cultures. The metabolite responsible for the activity was isolated by chromatographic techniques, purified and identified by spectroscopic methods as cyclosporine A. The antifungal activity against the pathogen was correlated with the presence of this metabolite by a dilution assay and then quantified. Cyclosporine A caused both growth inhibition and suppression of sclerotia formation. In a greenhouse assay, a significant increase in the number of surviving soybean (Glycine max) plants was observed when activity was isolated by chromatographic techniques, purified and identified by spectroscopic methods as cyclosporine A. The antifungal activity against the pathogen was correlated with the presence of this metabolite by a dilution assay and then quantified. Cyclosporine A caused both growth inhibition and suppression of sclerotia formation. In a greenhouse assay, a significant increase in the number of surviving soybean (Glycine max) plants was observed when S6 was determined in vitro by dual cultures. The metabolite responsible for the activity was isolated by chromatographic techniques, purified and identified by spectroscopic methods as cyclosporine A. The antifungal activity against the pathogen was correlated with the presence of this metabolite by a dilution assay and then quantified. Cyclosporine A caused both growth inhibition and suppression of sclerotia formation. In a greenhouse assay, a significant increase in the number of surviving soybean (Glycine max) plants was observed when activity was isolated by chromatographic techniques, purified and identified by spectroscopic methods as cyclosporine A. The antifungal activity against the pathogen was correlated with the presence of this metabolite by a dilution assay and then quantified. Cyclosporine A caused both growth inhibition and suppression of sclerotia formation. In a greenhouse assay, a significant increase in the number of surviving soybean (Glycine max) plants was observed when The antagonistic activity of Fusarium oxysporum strain S6 was determined in vitro by dual cultures. The metabolite responsible for the activity was isolated by chromatographic techniques, purified and identified by spectroscopic methods as cyclosporine A. The antifungal activity against the pathogen was correlated with the presence of this metabolite by a dilution assay and then quantified. Cyclosporine A caused both growth inhibition and suppression of sclerotia formation. In a greenhouse assay, a significant increase in the number of surviving soybean (Glycine max) plants was observed when activity was isolated by chromatographic techniques, purified and identified by spectroscopic methods as cyclosporine A. The antifungal activity against the pathogen was correlated with the presence of this metabolite by a dilution assay and then quantified. Cyclosporine A caused both growth inhibition and suppression of sclerotia formation. In a greenhouse assay, a significant increase in the number of surviving soybean (Glycine max) plants was observed when in vitro by dual cultures. The metabolite responsible for the activity was isolated by chromatographic techniques, purified and identified by spectroscopic methods as cyclosporine A. The antifungal activity against the pathogen was correlated with the presence of this metabolite by a dilution assay and then quantified. Cyclosporine A caused both growth inhibition and suppression of sclerotia formation. In a greenhouse assay, a significant increase in the number of surviving soybean (Glycine max) plants was observed whenGlycine max) plants was observed when S. sclerotiorum and F. oxysporum (S6) were inoculated together when compared with plants inoculated with S. sclerotiorum alone. with plants inoculated with S. sclerotiorum alone. and F. oxysporum (S6) were inoculated together when compared with plants inoculated with S. sclerotiorum alone.S. sclerotiorum alone. Conclusion: Fusarium oxysporum (S6) may be a good fungal biological control agent for S. sclerotiorum and cyclosporine A is the responsible metabolite involved in its antagonistic activity in vitro. involved in its antagonistic activity in vitro. agent for S. sclerotiorum and cyclosporine A is the responsible metabolite involved in its antagonistic activity in vitro. involved in its antagonistic activity in vitro. Fusarium oxysporum (S6) may be a good fungal biological control agent for S. sclerotiorum and cyclosporine A is the responsible metabolite involved in its antagonistic activity in vitro. involved in its antagonistic activity in vitro. S. sclerotiorum and cyclosporine A is the responsible metabolite involved in its antagonistic activity in vitro.in vitro. Significance and Impact of the Study: Cyclosporine A has not been previously described as an inhibitor of S. sclerotiorum. Its minimum inhibitory concentration (MIC) of 0Æ1 lg disc)1 makes it suitable to use as a biofungicide. In vivo (MIC) of 0Æ1 lg disc)1 makes it suitable to use as a biofungicide. In vivo described as an inhibitor of S. sclerotiorum. Its minimum inhibitory concentration (MIC) of 0Æ1 lg disc)1 makes it suitable to use as a biofungicide. In vivo (MIC) of 0Æ1 lg disc)1 makes it suitable to use as a biofungicide. In vivo Cyclosporine A has not been previously described as an inhibitor of S. sclerotiorum. Its minimum inhibitory concentration (MIC) of 0Æ1 lg disc)1 makes it suitable to use as a biofungicide. In vivo (MIC) of 0Æ1 lg disc)1 makes it suitable to use as a biofungicide. In vivo S. sclerotiorum. Its minimum inhibitory concentration (MIC) of 0Æ1 lg disc)1 makes it suitable to use as a biofungicide. In vivoÆ1 lg disc)1 makes it suitable to use as a biofungicide. In vivo experiments showed that F. oxysporum (S6) is a good candidate for the biocontrol of S. sclerotiorum in soybean. of S. sclerotiorum in soybean. F. oxysporum (S6) is a good candidate for the biocontrol of S. sclerotiorum in soybean.S. sclerotiorum in soybean.