Arabidopsis Protocols, Three Edition

SILVINA MANGANO, CINTIA DANIELA GONZALEZ SILVANA PETRUCCELLI.

Humana Press

Año: 2014 p. 9

978-1-62703-579-8

Transient assays provide a convenient alternative to stable transformation. Compared to the generation of stably transformed plants, agroinfiltration is more rapid, and samples can be analyzed a few days after inoculation. Nevertheless, at difference of tobacco and other plant species, Arabidopsis thaliana remains recalcitrant to routine transient assays. In this chapter, we describe a transient expression assay using simple infiltration of intact Arabidopsis thaliana leaves with Agrobacterium tumefaciens carrying a plasmid expressing a reporter fluorescence protein. In this protocol agrobacterium aggressiveness was increased by a prolonged treatment in the induction medium in presence of acetosyringone and deficient in nutrients. Besides, the arabidopsis plants were cultivated in intermediate period light cycle (12 h light-12 h dark) to promote leaf growth.