IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Early placental angiogenesis-vascularization and vegf/kdr receptor expression during mouse organogenesis after perigestational alcohol consumption.
Autor/es:
VENTUREIRA MR; SOBARZO CMA; NAITO M; ZANUZZI C; BARBEITO CG; CEBRAL E
Lugar:
Mar del Plata
Reunión:
Simposio; VI Latin American Symposium on Maternal-Fetal Interaction and Placenta and V Latin American Symposium on Reproductive Immunology Meeting.; 2015
Resumen:
The normal growth of the fetus depends on adequate placental development,  in  which  the  expression  of  trophoblast-decidual  VEGF  (vascular endothelial growth factor) conducts angiogenesis-vascularization. Previously, we found that perigestational alcohol consumption at mouse organogenesis (day 10 of gestation), causes embryo resorption, reduces decidualization and leads to histomorphological alterations of the decidual tissue. Objective: The aim was to study, after periconceptional alcohol intake, vascularization of early placentation by monitoring the number of uNK, VEGF and KDR-receptor expressions as well as KDR-activation, in murine decidua and labyrinth during organogenesis. Methods: Ethanol 10% was administered in the drinking water to CF-1 murine females (TF) for 17 days before and up to day 10 of gestation; water was administered to control females (CF). Labyrinthine development and decidual   (De)   vascularization   (H-E,   histochemistry   for   BSI-lectin, morphometry of decidual vascular area and labyrinth), the presence and number of uNKs (PAS and DBA-lectin) and the expression of VEGF and KDR (total and phosphorylated form) by immunohistochemistry, immunoforescence-confocal and western blot (WB) were analyzed in the implantation sites (IS). Results: Increased percentage of TF-IS had poor labyrinthine growth and reduced De-vascular lumen (p