1096 - MECHANISM OF LEPTIN ACTION ON THE INTRINSIC PROPERTIES OF PEDUNCULOPONTINE AND RETICULAR THALAMIC NEURONS

URBANO FJ; RIVERO ECHETO MC; BISAGNO V; BECK P; GARCIA-RILL E

RIO DE JANEIRO

Congreso; IBRO 2015 9th World Congress; 2015

IBRO

Obesity represents a major public health problem. Leptin, a hormone regulating appetite and energy expenditure, is increased in obese individuals. Leptin resistance is characterized by sleep/wake disturbances such as increased REM sleep, increased night-time arousals, and decreased percentage of total sleep. Several studies have shown that short sleep duration is highly correlated with decreased leptin levels in both animal and human models. Arousal and REM sleep are regulated by the cholinergic arm of the reticular activating system, the pedunculopontine nucleus (PPN) and its functional interaction with the reticular thalamic nucleus (Ret).We used whole cell patch recordings during application of leptin (100 nM) added to the artificial cerebrospinal fluid (aCSF) bathing slices from rats or ob/ob mice (and its corresponding C57BL/6 wildtype). During whole-cell patch clamp recording of PPN neurons, our results showed that leptin decreased action potential (AP) amplitude (n=28), AP frequency (n=10, p