IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
LOOKING FOR NEURONAL SUBSTRATES OF MEMORY
Autor/es:
HEPP, Y; FREUDENTHAL, R; MALDONADO, H; PEDREIRA, ME
Lugar:
INIBIBB-CCT, Bahía Blanca, Buenos Aires, Argentina
Reunión:
Workshop; Workshop International, Sociedad Argentina de Investigación en Neurociencias; 2008
Institución organizadora:
SAN
Resumen:
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A topic of interest in
neurosciences is to determine the function of different areas of the nervous
system during memory formation. In different vertebrate models the technique
employed aimed to this subject consists of the use of microinjections of
neurotoxic drugs as the glutamatergic agonist ibotenic acid that causes
neuronal destruction in the injection area. My project is focus on the development
of a local microinjection technique in an invertebrate model of memory. In our
laboratory we have been developing pharmacological experiments carried out in
the model of associative context-signal long term memory in the Chasmagnathus granulatus crab. These
experiments consist of the systemic administration of different drugs which
allow us to discern the molecular events that underlie consolidation,
reconsolidation and extinction. Therefore, the local microinjections technique
allows us to administrate neurotoxic drugs in areas on the nervous system of
the crabs which are potentially involved in memory processes. The first step
consists of setting up a stereotaxic map of the nervous system of the crab.
Until now, the results show that it would be possible to generate a coordinates
system of reference for the eyestalks and the supraesophageal ganglion of the
crab. The references of this coordinates system are
external anatomical features as the pseudopupil, the ommatidial edge, the
cuticle and the exoskeleton frontal plaque. By confocal microscopy after
microinjections with propidium iodide in the selected area, it could be
possible to determine the injection area and to know the scope of the injection
in the afected area. By immunohistochemistry we observe
an NMDA like receptor signal with a broad distribution all
over the structures analyzed. Therefore, the
ibotenic acid microinjection will give us the opportunity to perform localized
lesions and to design experiments aimed to establish a correlation between the
zones injured and the behavioral changes observed.