IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Expression and activity of matrix metalloprotease-2 in canine early placenta
Autor/es:
CEBRAL E; DIESSLER M; VENTUREIRA, MR; SOBARZO C; HERNANDEZ R; CASAS L; BARBEITO C
Lugar:
Mar del Plata
Reunión:
Simposio; VI SLIMP and V LASRI. Maternal-Fetal Interaction; 2015
Institución organizadora:
SLIMP
Resumen:
ObjetiveImplantation and placentation requiretissue remodeling through enzymes of matrix metalloproteasesgroup (MMPs). Because the expression of these enzymes has been studied inimplantation and placentation of species with different types of placentae, butnot in the canine endotheliochorial one, the objective of this work was toinvestigate the expression and activity of MMP-2 in placenta of canine at theearliest gestational stage.MethodsSamples from four early canine placentae(18-30 days) were obtained. The placentae from one uterine horn of each bitchwere formalin-fixed, while those from the other horn were obtained on dry iceand kept at -70° for molecular determinations. Slices were routined colouredand processed by PAS-reaction to analyze general features. MMP-2 expression wasanalyzed by immunohistochemistry (IHC) and Western Blot (WB), and enzymeactivity was evaluated by zymography (Zg). Maternal, labyrinthine andhaematomal zones were analyzed in each case. Resultsand ConclusionsPositive reaction to the MMP-2 antibody wasobserved in the labyrinth and haematoma, being stronger in cytotrophoblastcells and syncytium. WB showed a MMP-2 band between 53-68 kDa in labyrinth (L),haematoma (H), implantational endometrium (IE) and non-implantationalendometrium (No-IE). Laberynthine MMP-2 level of expression (mean OD/actin ±SD)) was slightly higher than haematomal MMP-2 (p<0,05), whereas IE MMP-2expression had the lowest level. The relation L/IE was 2.5 and H/IE 1.8. By Zg,it was detected only the active MMP-2 at 54 kDa, both in L and H. However,laberynthine had lower MMP-2 activity than MMP-2 one of haematoma (p<0,05).These data show that MMP-2 expression and activation is dependent of theplacental tissue, and they suggest that it actively participates in aspecific tissue remodeling that takes place during early placentation in canines.