IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Active G protein reports the fraction of occupied receptors in the pheromone pathway of yeast
Autor/es:
ALAN BUSH; ALEJANDRO COLMAN-LERNER
Lugar:
Rosario - Santa Fe
Reunión:
Congreso; L Reunión anual de la Sociedad de investigadores en Bioquímica y Biología molecular (SAIB); 2014
Institución organizadora:
SAIB
Resumen:
Receptor theory postulates that cells responds to extracellular ligand concentration through the formation of a receptor-ligand complex, and transduce information of the amount of this complex to downstream effectors. A simple prediction of this model is that modifications in the abundance of receptors should modify the dose response curve. We tested this prediction in the pheromone response pathway of the yeast Saccharomyces cerevisiae, and found no differences in the transcriptional response when the levels of the receptor were varied. We developed a detailed mathematical model of this system, which includes the activation of the heterotrimeric G protein and its coupling to the receptor. Analysis of the model suggested a mechanism by which the response can be robust to changes in the total amount of receptors. Our model is consistent with many results from the literature that are hard to explain by other mechanisms. We are currently working on further experiment to test the predictions of our model.