IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Light, chromatin and alternative splicing
Autor/es:
GODOY HERZ, MICAELA A; PETRILLO, EZEQUIEL; KORNBLIHTT, ALBERTO
Reunión:
Congreso; SAIB, 50th Annual Meeting, Argentine Society for Biochemistry and Molecular Biology; 2014
Resumen:
Light is not only a source of energy but also a key regulator of plant physiological adaptations. We have previously shown that light/dark conditions affect alternative splicing of the pre-mRNA for At-RS31, a Ser-Arg-rich splicing factor. This led us to investigate whether chromatin modifications play a role in this light regulation of alternative splicing. Increasing concentrations of trichostatin A (TSA), a drug that suppresses histone deacetylase activity and therefore increases histone acetylation, mimic the effect of light on At-RS31 alternative splicing in a dose-dependent manner. The light/dark effect and the TSA effect on alternative splicing are not observed in the pre-mRNA for At-RS2Z33, another SR-splicing factor used as a control. Global histone acetylation does not seem to change between light and dark treated plants. Using Arabidopsis mutants defective in different histone deacetylases we found that the light/dark effect is strongly reduced in hd1 mutants. In contrast, the light/dark effect is still observed in hda15 mutants, suggesting a mechanism that involves specific histone modification enzymes. Further experiments such as a chromatin inmunoprecipitation assay of chromatin marks will be carried out in the future to determine the link between chromatin modifications and alternative splicing regulation by light.