Acid-sensing ion channel 1A (ASIC1A) and its gender dependent role in neuromuscular synaptic transmission

LINO NG; URBANO FJ; UCHITEL OD

HUERTA GRANDE, CORDOBA ARGENTINA

Congreso; XXVIII CONGRESO ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN EN NEUROCIENCIAS. REUNIÓN SATELITE/NEUROBIOLOGÍA DEL COMPORTAMIENTO: NEUROETOLOGÍA y NEUROBIOLOGÍA DE LA MEMORIA EN EL CONO SUR; 2013

SOCIEDAD ARGENTINA DE INVESTIGACIÓN EN NEUROCIENCIAS

Synaptic Transmission and Excitability. Poster Number 220 / Session 1------------------------------- CNS changes in extracellular pH (pHO) have considerable influence on the physiology of neurons. At the neuromuscular junction, H+ are co-release with Ach and are pump into the synaptic cleft after prolonged stimulation, contributing to muscle fatigue. Since acid-sensing ion channels (ASICs) have been recently identified and proposed to regulate neuromuscular synaptic transmission in response to acidosis, our aim was to investigate the modulatory role of ASIC1a on neuromuscular transmission and its dependence on gender. For that purpose, we initially studied the frequency of MEPPs in the absence or presence of either TTX or ASICs modulators. In males, we observed a considerable decrease in the frequency of MEPPs as a consequence of the genetic removal of ASIC1a. On the contrary, the frequency in females was significantly increased in KOs (P