Acid-sensing ion channel 1A (ASIC1A) and its genderdependent role in neuromuscular synaptic transmission

LINO NOELIA G.; F. J. URBANO; UCHITEL OD.

Huerta Grande. Cordoba

Congreso; XXVIII CONGRESO ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACION EN NEUROCIENCIAS; 2013

SOCIEDAD ARGENTINA DE INVESTIGACION EN NEUROCIENCIAS

Synaptic Transmission and Excitability Poster Number 220 / Session 1 Acid-sensing ion channel 1A (ASIC1A) and its genderdependent role in neuromuscular synaptic transmission Noelia G. Lino, Franscisco J Urbano, Osvaldo D Uchitel Instituto de Fisiología, Biología Molecular y Neurociencias, IFIBYNE UBA-CONICET, Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina linonoelia@hotmail.com ________________________________________________________ CNS changes in extracellular pH (pHO) have considerable influence on the physiology of neurons. At the neuromuscular junction, H+ are co-release with ACh and are pump into the synaptic cleft after prolonged stimulation, contributing to muscle fatigue. Since acid-sensing ion channels (ASICs) have been recently identified and proposed to regulate neuromuscular synaptic transmission in response to acidosis, our aim was to investigate the modulatory role of ASIC1a on neuromuscular transmission and its dependence on gender.For that purpose, we initially studied the frequency of MEPPs in the absence or presence of either TTX or ASICs modulators.In males, we observed a considerable decrease in the frequency of MEPPs as a consequence of the genetic removal of ASIC1a. On the contrary, the frequency in females was significantly increased in KOs (P