Functional role of peroxisome proliferator-activated receptors gamma in glucidic metabolism in of granulosa cells from normal and polycystic ovary syndrome women

SALINAS A; PUSTOVRH C; GODOY A; ARGUELLO B; MUÑOZ A; KOHEN P; DEVOTO L

Congreso; ISGE_2012 XV WORLD CONGRESS OF GYNECOLOGICAL ENDOCRIONOLOGY; 2012

Functional role of peroxisome proliferator-activated receptors gamma in glucidic metabolism in of granulosa cells from normal and polycystic ovary syndrome women. Salinas Abril, Pustovrh M. Carolina, Godoy Ana, Argüello Begonia, Muñoz Alex, Kohen Paulina, Devoto Luigi. Institute of Maternal and Child Research.(IDIMI) Department of Obstetrics and Gynecology. Hospital San Borja Arriarán. Faculty of Medicine University of Chile. Santiago Chile Polycystic ovary syndrome (PCOS) is one of the most common endocrine and metabolic disorders that affect 5-10% of women during reproductive age. In addition 40 to 50% of these patients exibit hyperinsulinemia and insulin resistance (IR). Adipocytes and muscle cells of PCOS women have a reduced uptake of glucose and expression of glucotransporters, without changes in receptor number or affinity for insulin.(I) It is thought that IR of these tissues is associated with impaired post-receptor signaling.PPARγ are key transcription factors that regulate metabolism of glucose and I. Rosiglitazone (Rz) belongs to the family of thiazolidinediones, it is a potent selective agonist of PPARγ receptors, regulating the expression of genes involved in lipid and glucose metabolism. Glitazones increase the incorporation of glucose-insulin-dependent in cultured myocytes and adipocytes modulating the expression of GLUTs. Conversely, the glucose metabolism and the insulin signaling pathway in granulosa cells (GCs) of PCOS patients are virtually unknown. The aim of this study was to determine the role of PPARγ and its agonist (Rz) in the metabolic pathway of I in granulosa cells of PCOS patients. Methodology: Follicular fluid (FF) and GCs were collected from PCOS ( n= 15) patient participating in the IVF program of our institute (IDIMI) Similar samples were collected from normal women involved in our IVF due to male factor infertility and were used as control group ( n= 18 ). GLUTs and PPAR γ genes expression were evaluated by qRT-PCR, The proteins were localize and determined by immunocistochemistry, (ICQ) inmunofluorescence and Western blot. Glucose was determined in FF by glucose oxidase technique and its uptake was assessed by incorporation of 2-desoxy-3H-glucose (2-DG) in both basal and stimulated condition I (100 ng/ml), as well as in cells pre incubated with Rz (0,01-1 μM).Results: Glucose levels in follicular fluid of PCOS follicles were significant higher compared to the control group (41,14 ± 5,1 v/s 18,33 ± 2,8 mg/dl p