Synaptic NF-kappa B localization dynamics in learning and memory

ANGELES SALLES; MARIA LAGOS; MARIANO BOCCIA; MARIANO BLAKE; CARLOS MARIA BARATTI; NICOLETTA CORBI; CLAUDIO PASSANATI; ARTURO ROMANO ; RAMIRO FREUDENTHAL

Huerta Grande

Congreso; XXVII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2012

San

NF-kappa B activation has been shown to be necessary for long-term memory consolidation in the mouse inhibitory avoidance learning paradigm. NF-kappa B is activated in the nucleus of hippocampal cells in a specific temporal window during consolidation. Previous results showed that the transcription factor is also present in synaptic terminals. More importantly we found NF-kappa B strongly bound to membranes of these terminals. This new finding poses the question, is there a local role for NF-kappa B? And if so, is it essential for learning and memory? Another pool of NF-kappa B has been found in the synaptosomal content, and its activation measured through Electro Mobility Shift Assays (EMSA) is modified during consolidation of shocked mice, when comparing to naïve and unshocked. The membrane bound NF-kappa B increases 5 minutes post training only in shocked mice. The re localization of the transcription factor could implicate a new function of NF-kappa B that might intervene in the consolidation process. The aim of this research is to study the dynamics of synaptic NF-kappa B activation during consolidation, discussing the novel localization of the transcription factor in membranes of synaptic terminals.