IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
5-Bromo-2`deoxyuridine is toxic to olfactory epithelium cells at concentrations widely used for studies of cells lineage
Autor/es:
RAICES, M; JUNGBLUT, L D; PAZ, D A; POZZI, A G
Lugar:
Huerta Grande, Córdoba
Reunión:
Congreso; XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; 2011
Institución organizadora:
Sociedad Argentina de Investigación en Neurociencias
Resumen:
The 5-bromo-2´-deoxyuridine (BrdU), is a thymidine analogue that incorporates in to the DNA of dividing cells during the S-phase of the cell cycle. This analogue is widely used for the study of mitosis and cell lineage in vitro and in vivo. However, there are reports that questioned the validity of its use in some biological systems where BrdU showed cellular toxicity. The current work examines the effect of BrdU incorporation on the phenotype of cells in the olfactory epithelium (OE). We found a decrease in mature olfactory receptor neurons (ORNs) as a function of BrdU exposition time when we used 10 mM BrdU. We also observed an increase in the number of apoptotic cells after the BrdU administration. Moreover, while the epithelial structure in BrdU treated animals was maintained, the histochemical analysis showed alterations in secretory vesicles from the sustentacular cells. BrdU 0,1-1 mM did not show the same toxic effects. We conclude that the use of BrdU 10 mM (doses of current use in the literature) has toxic effects on ORNs, activating classical cell death pathways and altering the histomorphology of the sustentacular cells. These results demonstrate the importance of using the correct dose of BrdU in neurogenesis studies both in vivo and in vitro.