IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
To labilize or not to labilize? The importance of being proteasome
Autor/es:
MARÍA SOL FUSTIÑANA; VERÓNICA DE LA FUENTE; RAMIRO FREUDENTHAL; ARTURO ROMANO
Lugar:
Huerta Grande
Reunión:
Congreso; XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; 2011
Institución organizadora:
San
Resumen:
Once a memory is consolidated, the presentation of a reminder can induce reconsolidation, a process that has inherent stages: reactivation, labilización and restabilization of the memory trace. We are studying the labilization process in two models: context-signal memory in crab and fear conditioning in mouse. A previous study showed that the labilization process involved protein degradation by the proteasome. We found that the inhibition of the proteasome by MG132, block labilization. This drug can inhibit NF-kB during consolidation and this inhibition results in an amnesic effect but, when we administered MG132 previous the re-exposure we did not find an amnesic effect. When we co-injected MG132 with a NF-kB inhibitor we could not find the amnesic effect we expected. The same result was obtained when we co-injected MG132 with a NMDA receptor inhibitor. The GABA inhibitor bicuculline can enhance memory reconsolidation in the crab paradigm, but this effect was not found when it was co-injected with MG132. All together these results indicate that proteasoma is involved in memory labilization. We hypothesized that memory labilization implies partial synapses retraction involving proteasoma dependent protein degradation and then stabilization during the reconsolidation process.